Voltage-independent barium-permeable channel activated in Lymnaea neurons by internal perfusion or patch excision.
Voltage-independent barium-permeable channel activated in Lymnaea neurons by internal perfusion or patch excision.
复制标题
通过内部灌注或补片切除激活 Lymnaea 神经元中的电压依赖性钡渗透通道。
DOI：
10.1007/bf01871084
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Byerly,L
中科院分区：
文献类型：
--
作者：
Yazejian,B;Byerly,L
Isolated nerve cells fromLymnaea stagnaliswere studied using the internal-perfusion and patch-clamp techniques. Patch excision frequently activated a voltage-independent Ba2+-permeable channel with a slope conductance of 27 pS at negative potentials (50mmBa2+). This channel is not seen in patches on healthy cells and, unlike the voltage-dependent Ca channel, is not labile in isolated patches. The activity of the channel in inside-out patches is unaffected by intracellular ATP, Ca2+below 1mmor the catalytic subunit of cAMP-dependent protein kinase but is reversibly blocked by millimolar intracellular Ca2+or Ba2+. The channel can be activated in on-cell patches by either internal perfusion with high Ca2+or the long-term internal perfusion of low Ca2+solutions not containing ATP. These channels may carry the inward Ca2+current which causes a regenerative increase in intracellular Ca+when snail neurons are perfused with high Ca2+solutions. High internal Ca2+, or long periods of internal perfusion with ATP-free solutions, induces an increase in a resting (−50 mV) whole-cell Ba2+conductance. This conductance can be turned off by returning the intracellular perfusate to a low Ca2+solution containing ATP and Mg2+. The activity of this channel appears to have an opposite dependence on intracellular conditions to that of the voltage-dependent Ca channel.
