A microbiome engineering framework to evaluate rhizobial symbionts of legumes
A microbiome engineering framework to evaluate rhizobial symbionts of legumes
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DOI：
10.1007/s11104-021-04892-2
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发表时间：
2021-03-04
期刊：
影响因子：
4.9
通讯作者：
Atamian, Hagop S.
中科院分区：
文献类型：
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作者：
Quides, Kenjiro W.;Atamian, Hagop S.
背景世纪以来，根瘤菌一直被认为是豆科作物的有效生物肥料选择。这导致了根瘤菌接种剂在农业系统中的广泛使用，但出现了一个反复出现的问题：应用根瘤菌难以为豆类作物提供生长益处。这在很大程度上归因于土壤根瘤菌的存在，并被称为“根瘤菌竞争问题”。微生物组工程已经成为一种通过创造不需要外源根瘤菌的豆类微生物组来规避根瘤菌竞争问题的方法。然而，我们强调了微生物组工程的另一种实施方式，其重点是解开导致根瘤菌竞争问题的共生关系的复杂性。我们概述了三种使用不同起始接种物来测试假设以克服根瘤菌竞争问题的方法。结论我们建议的方法是针对不同阶段的豆科植物根瘤菌共生，并将帮助我们发现潜在的分子机制，有助于根瘤菌的竞争问题。最后，我们对这些不同的方法进行了综合分析，并为豆类及其复杂的微生物组的未来研究提出了一条前进的道路。
Background For well over a century, rhizobia have been recognized as effective biofertilizer options for legume crops. This has led to the widespread use of rhizobial inoculants in agricultural systems, but a recurring issue has emerged: applied rhizobia struggle to provide growth benefits to legume crops. This has largely been attributed to the presence of soil rhizobia and has been termed the 'rhizobial competition problem.' Scope Microbiome engineering has emerged as a methodology to circumvent the rhizobial competition problem by creating legume microbiomes that do not require exogenous rhizobia. However, we highlight an alternative implementation of microbiome engineering that focuses on untangling the complexities of the symbiosis that contribute to the rhizobial competition problem. We outline three approaches that use different starting inocula to test hypotheses to overcome the rhizobial competition problem. Conclusions The approaches we suggest are targeted at various stages of the legume-rhizobium symbiosis and will help us uncover underlying molecular mechanisms that contribute to the rhizobial competition problem. We conclude with an integrative perspective of these different approaches and suggest a path forward for future research on legumes and their complex microbiome.