Differential control of cell affinity required for progression and refinement of cell boundary during Drosophila leg segmentation
Differential control of cell affinity required for progression and refinement of cell boundary during Drosophila leg segmentation
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DOI：
10.1016/j.ydbio.2007.07.001
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发表时间：
2007-09-01
影响因子：
2.7
通讯作者：
Hayashi, Shigeo
中科院分区：
文献类型：
--
作者：
Sakurai, Kayoko T.;Kojima, Tetsuya;Hayashi, Shigeo
发育过程中区域边界的形成涉及将不同类型的细胞分成不同的空间域。果蝇腿的膝盖骨5(Ta5)和膝前(Pre)之间的节段边界最初出现在腿盘的中心，并逐渐变得尖锐和扩展到其最终位置，伴随着细胞识别分子Caprous和Tartan的下调以及细胞从Ta5到Pre的跨越边界的移位。反复无常和鞑靼由转录因子Bar和Al控制，它们的功能丧失导致细胞与野生型邻居的亲和力降低和细胞置换活性。此外，虽然突变细胞形成了Ta5/Pre边界，但其进展和锐化受到了影响。过度表达反复无常或格子花纹的细胞在Ta5和Pre内变得侵袭，有时逃避细胞运动的隔间限制。由反复无常和Tartan介导的细胞亲和力的动态时空调节是改善Ta5/Pre边界的关键特性。(C)2007 Elsevier Inc.保留所有权利。
Domain boundary formation in development involves sorting of different types of cells into separate spatial domains. The segment boundary between tarsus 5 (Ta5) and the pretarsus (Pre) of the Drosophila leg initially appears at the center of the leg disc and progressively sharpens and expands to its final position, accompanied by down-regulation of the cell recognition molecule Capricious and Tartan and cell displacement from Ta5 to Pre across the boundary. Capricious and Tartan are controlled by transcription factor Bar and Al, and their loss of function leads to reduction of cell affinity to wild type neighbors and cell displacement activities. In addition, although the mutant cells formed Ta5/Pre boundary, its progression and sharpening were compromised. Cells overexpressing Capricious or Tartan became invasive within Ta5 and Pre, sometimes escaping the compartmental restriction of cell movement. Dynamic spatiotemporal regulation of cell affinity mediated by Capricious and Tartan is a key property of refinement of the Ta5/Pre boundary. (c) 2007 Elsevier Inc. All rights reserved.