Extensive molecular differences between anterior- and posterior-half-sclerotomes underlie somite polarity and spinal nerve segmentation
Extensive molecular differences between anterior- and posterior-half-sclerotomes underlie somite polarity and spinal nerve segmentation
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DOI：
10.1186/1471-213x-9-30
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发表时间：
2009-05-22
影响因子：
--
通讯作者：
Tannahill, David
中科院分区：
文献类型：
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作者：
Hughes, Daniel S. T.;Keynes, Roger J.;Tannahill, David
背景：somite衍生的硬核分化为前半部分和后半部分是椎体形态发生和脊神经分割的基础。为了全面描述这种极性背后的分子差异，我们对小鼠胚胎中两个核组之间的基因表达进行了系统的比较。结果：数百个基因在两个核组之间存在差异表达，表明分子异质性的显著程度支撑着体体极性的发展。结论：我们已经确定了一组值得进一步研究的基因，它们是体体极性和椎体形态发生的调节因子，以及脊髓轴突生长的排斥因子。此外，研究结果表明，与后半硬核组不同，前半硬核组的中央区域不为脊柱提供骨和软骨，而是与节段脊神经的发育有关。
Background: The polarization of somite-derived sclerotomes into anterior and posterior halves underlies vertebral morphogenesis and spinal nerve segmentation. To characterize the full extent of molecular differences that underlie this polarity, we have undertaken a systematic comparison of gene expression between the two sclerotome halves in the mouse embryo.Results: Several hundred genes are differentially-expressed between the two sclerotome halves, showing that a marked degree of molecular heterogeneity underpins the development of somite polarity.Conclusion: We have identified a set of genes that warrant further investigation as regulators of somite polarity and vertebral morphogenesis, as well as repellents of spinal axon growth. Moreover the results indicate that, unlike the posterior half-sclerotome, the central region of the anterior-half-sclerotome does not contribute bone and cartilage to the vertebral column, being associated instead with the development of the segmented spinal nerves.