Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts
Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts
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DOI：
10.1038/382420a0
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发表时间：
1996-08-01
期刊：
影响因子：
64.8
通讯作者：
Karsenti, E
中科院分区：
文献类型：
--
作者：
Heald, R;Tournebize, R;Karsenti, E
功能性细胞核和有丝分裂纺锤体组装在非洲爪蟾卵提取物中孵育的DNA包被珠周围。在没有中心体和动粒的情况下，双极纺锤体组装，表明双极性是有丝分裂细胞质中微管围绕染色质组装的固有特性。微管在分散的位点以随机极性成核，重新排列成两个极性一致的阵列。纺锤极的形成需要细胞质动力蛋白依赖的微管相互易位。有人提出，纺锤体的形式在没有中心体的马达依赖性排序微管根据其极性。
Functional nuclei and mitotic spindles are shown to assemble around DNA-coated beads incubated in Xenopus egg extracts. Bipolar spindles assemble in the absence of centrosomes and kinetochores, indicating that bipolarity is an intrinsic property of microtubules assembling around chromatin in a mitotic cytoplasm. Microtubules nucleated at dispersed sites with random polarity rearrange into two arrays of uniform polarity. Spindle-pole formation requires cytoplasmic dynein-dependent translocation of microtubules across one another. It is proposed that spindles form in the absence of centrosomes by motor-dependent sorting of microtubules according to their polarity.