Cloning, expression, and chromosomal localization of the rat mitochondrial capsule selenoprotein gene (MCS), the reading frame does not contain potential UGA selenocysteine codons
Cloning, expression, and chromosomal localization of the rat mitochondrial capsule selenoprotein gene (MCS), the reading frame does not contain potential UGA selenocysteine codons
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DOI：
10.1089/dna.1996.15.159
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发表时间：
1996-02-01
影响因子：
3.1
通讯作者：
Engel, W
中科院分区：
文献类型：
--
作者：
Adham, IM;Tessmann, D;Engel, W
线粒体包膜硒蛋白（MCS）是一种含硒多肽。它是维持和稳定精子线粒体新月形结构的三种重要蛋白质之一。本文报道了大鼠MCS cDNA和基因的分离和鉴定。该cDNA含有一个145个氨基酸的蛋白质的阅读框架，并且它缺少UGA密码子，该密码子已在小鼠MCS cDNA的阅读框架中发现，并且已被推测在推导的小鼠氨基酸序列的氨基末端编码硒代半胱氨酸。推导的大鼠和小鼠MCS的氨基酸序列显示出高水平的同源性（79%）。大鼠MCS基因包含两个外显子;内含子序列在与小鼠MCS基因相同的位置中断5'非翻译序列。转录起始位点位于翻译起始位点上游184 bp处。小鼠和大鼠基因的5 '侧翼区的比对显示，转录起始位点上游的前400个核苷酸表现出73%的总体序列相似性。该保守区不含TATA或CAAT盒基序。北方印迹分析表明，MCS mRNA仅在生后发育30天后的睾丸中可检测到。此外，原位杂交显示，大鼠MCS基因主要表达在圆形精子细胞。从分离大鼠染色体的小鼠-大鼠细胞杂交的分析，MCS基因被分配到大鼠2号染色体。
The mitochondrial capsule selenoprotein (MCS) is a selenium-containing polypeptide. It is one of three proteins that are important for the maintenance and stabilization of the crescent structure of the sperm mitochondria. In this paper, we report the isolation and characterization of the rat MCS cDNA and gene. The cDNA contains a reading frame for a 145-amino-acid protein and it lacks the UGA codons, which have been found in the reading frame of the mouse MCS cDNA and have been presumed to encode the selenocysteine in the amino terminal of the deduced mouse amino acid sequence. The deduced amino acid sequence of the rat and mouse MCS shows a high level of homology (79%). The rat MCS gene contains two exons; the intron sequence interrupts the 5' untranslated sequence at the same position as in the mouse MCS gene. The transcription start site is located 184 bp upstream of the translation start site. Alignment of the 5'-flanking regions of the mouse and rat genes reveals that the first 400 nucleotides upstream of the transcription start site exhibit an overall sequence similarity of 73%. This conserved region contains no TATA or CAAT box motifs. Northern blot analysis indicates that the MCS mRNA is detectable only in the testis after day 30 of postnatal development. Moreover, in situ hybridization revealed that the rat MCS gene is mainly expressed in round spermatids. From the analysis of mouse-rat cell hybrids that segregate rat chromosomes, the MCS gene was assigned to rat chromosome 2.