Losartan and its metabolite E3174 modify cardiac delayed rectifier K+ currents
Losartan and its metabolite E3174 modify cardiac delayed rectifier K+ currents
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DOI：
10.1161/01.cir.101.10.1199
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发表时间：
2000-03-14
期刊：
影响因子：
37.8
通讯作者：
Tamargo, J
中科院分区：
文献类型：
--
作者：
Caballero, R;Delpón, E;Tamargo, J
背景1型血管紧张素II受体拮抗剂氯沙坦及其代谢产物E3174对跨膜动作电位，hKv1.5，HERG和I-Ks电流的影响进行了分析。方法和结果豚鼠心室动作电位记录与微电极技术和hKv1.5和HERG电流与全细胞膜片钳技术。用穿孔制霉菌素贴片记录豚鼠心室肌细胞的I-Ks。氯沙坦和E3174分别使hKv1.5电流瞬时增加8.0 +/- 1.4%和7.4 +/-1.6%。此后，它们产生电压依赖性阻滞，E3174比氯沙坦更有效（P
Background-The effects of type 1 angiotensin II receptor antagonist losartan and its metabolite E3174 on transmembrane action potentials, hKv1.5, HERG, and I-Ks currents were analyzed.Methods and Results-Guinea pig ventricular action potentials were recorded with microelectrode techniques and hKv1.5 and HERG currents with the whole-cell patch-clamp technique. I-Ks was recorded in guinea pig ventricular myocytes with the perforated-nystatin-patch configuration. Losartan and E3174 transiently increased the hKv1.5 current by 8.0 +/- 1.4% and 7.4 +/- 1.6%, respectively. Thereafter, they produced a voltage-dependent block, E3174 being more potent than losartan (P