Physicochemical Changes of Few-Layer Graphene in Peroxidase-Catalyzed Reactions: Characterization and Potential Ecological Effects
Physicochemical Changes of Few-Layer Graphene in Peroxidase-Catalyzed Reactions: Characterization and Potential Ecological Effects
复制标题
过氧化物酶催化反应中少层石墨烯的物理化学变化：表征和潜在的生态效应
DOI：
10.1021/acs.est.5b02261
复制
发表时间：
2015-07-21
影响因子：
11.4
通讯作者：
Mao, Liang
中科院分区：
文献类型：
--
作者：
Lu, Kun;Huang, Qingguo;Mao, Liang
The environmental implications of graphene have received much attention, however, little is known about how graphene affects or may be affected by the enzymatic reactions that are critically involved in natural organic Matter transformation processes.. We conducted experiments to examine the role of few-layer graphene (FLG) in the reaction system of tetrabromobisphenol A (TBBPA) mediated by horseradish peroxidase (HRP). We found that TBBPA was transformed by REP into two products that were likely formed from coupling of two TBBPA radicals via interaction of an oxygen atom on one radical and a, propyl-substituted aromatic carbon atom on the other. Presence of FLG greatly increased the reaction rate by protecting REP from inactivation. Direct reactions between TBBPA radicals and FLG were unequivocally evidenced using C-14 labeling and the characteristic photoelectron response of bromine contained in TBBPA. The thickness, size, and aggregation profile of FLG was modified by the reaction as shown by multiple characterization tools. Assessment using Daphnia magna revealed a substantial decrease in the bioaccumulation and toxicity of the FLG after being modified. The data provides the first evidence that FLG can be modified in REP-mediated reactions and indicates that such modifications may have strong implications in its ecological effects.
