PHOSPHOLIPID VESICLE FORMATION AND TRANSMEMBRANE PROTEIN INCORPORATION USING OCTYL GLUCOSIDE
PHOSPHOLIPID VESICLE FORMATION AND TRANSMEMBRANE PROTEIN INCORPORATION USING OCTYL GLUCOSIDE
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DOI：
10.1021/bi00507a028
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发表时间：
1981-01-01
期刊：
影响因子：
2.9
通讯作者：
REYNOLDS, JA
中科院分区：
文献类型：
--
作者：
MIMMS, LT;ZAMPIGHI, G;REYNOLDS, JA
从[鸡]蛋黄磷脂酰胆碱和辛基葡糖苷的混合胶束中去除洗涤剂导致形成直径约为230 nm的单层磷脂囊泡。同样的程序适用于混合胶束含有[人红细胞]跨膜蛋白血型糖蛋白A，除了脂质和去污剂，产生囊泡的大小相同的血型糖蛋白纳入双层。纯脂质囊泡对阴离子和阳离子都是高度不可渗透的，并且并入多达220个分子的血型糖蛋白/囊泡对渗透性几乎没有影响。
Removal of detergent from mixed micelles of [hen] egg yolk phosphatidylcholine and octyl glucoside leads to formation of unilamellar phospholipid vesicles with a diameter of about 230 nm. The same procedure applied to mixed micelles containing the [human erythrocyte] transmembrane protein glycophorin A, in addition to lipid and detergent, produces vesicles of the same size with glycophorin incorporated into the bilayer. The pure lipid vesicles are highly impermeable to both anions and cations, and incorporation of up to 220 molecules of glycophorin/vesicle has little effect on permeability.