Characterization of novel stem cell factor responsive human mast cell lines LAD 1 and 2 established from a patient with mast cell sarcoma/leukemia;: activation following aggregation of FcεRI or FcγRI
Characterization of novel stem cell factor responsive human mast cell lines LAD 1 and 2 established from a patient with mast cell sarcoma/leukemia;: activation following aggregation of FcεRI or FcγRI
复制标题
DOI：
10.1016/s0145-2126(02)00343-0
复制
发表时间：
2003-08-01
影响因子：
2.7
通讯作者：
Metcalfe, DD
中科院分区：
文献类型：
--
作者：
Kirshenbaum, AS;Akin, C;Metcalfe, DD
从肥大细胞肉瘤/白血病患者的骨髓穿刺液中建立了两种新的依赖于斯坦细胞因子（SCF）的人肥大细胞系，命名为LAD I和2。LAD 1和2细胞具有人肥大细胞的超微结构特征，并表达Fc ε RI、CD 4、9、13、14、22、31、32、45、64、71、103、117、132、CXCR 4（CD 184）、CCR 5（CD 195）;以及胞浆内组胺、类胰蛋白酶和糜蛋白酶。LAD I和2在c-kit的密码子816处不显示激活突变。LAD I和2在Fc ε RI或Fc γ RI聚集后均释放β-氨基己糖苷酶。这些细胞系的可用性为研究人类肥大细胞的生物学提供了无与伦比的环境。
Two novel stein cell factor (SCF) dependent human mast cell lines, designated LAD I and 2, were established from bone marrow aspirates from a patient with mast cell sarcoma/leukemia. LAD1 and 2 cells have the ultrastructural features of human mast cells, and express FcepsilonRI, CD4, 9, 13, 14, 22, 31, 32, 45, 64, 71, 103, 117, 132, CXCR4 (CD 184), CCR5 (CD 195); and intracytoplasmic histamine, tryptase and chymase. LAD I and 2 do not exhibit activating mutations at codon 816 of c-kit. Both LAD I and 2 release beta-hexosaminidase following FcepsilonRI or FcgammaRI aggregation. The availability of these cell lines offers an unparalleled circumstance to examine the biology of human mast cells.