Interaction of poliovirus-encoded 2C/2BC polypeptides with the 3′ terminus negative-strand cloverleaf requires an intact stem-loop b
Interaction of poliovirus-encoded 2C/2BC polypeptides with the 3′ terminus negative-strand cloverleaf requires an intact stem-loop b
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DOI：
10.1006/viro.2000.0770
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发表时间：
2001-02-01
期刊：
影响因子：
3.7
通讯作者：
Dasgupta, A
中科院分区：
文献类型：
--
作者：
Banerjee, R;Tsai, WM;Dasgupta, A
脊髓灰质炎病毒编码的膜相关多肽2C及其前体2BC被认为是病毒RNA合成起始和延伸所需的。先前的研究表明，2C多肽特异性地与脊髓灰质炎病毒负链RNA的3 '末端序列相互作用。这种相互作用是由保守序列UGUUUU的存在下，在“茎a”内的3 '-末端三叶草结构，我们表明，2C前体。2BC也与负链RNA的3 '末端三叶草相互作用。我们还证明了2C/2BC与负链3 '端序列的相互作用不仅取决于含有UGUUUU序列的完整“茎a”，而且同样受到负链三叶草内完整“茎B”的影响，本文的结果表明，茎a UGUUUU序列相对于完整茎b的空间构型对2C/2BC与3 '-末端负链三叶草结构的相互作用。(C)北京：科学出版社.
The poliovirus-encoded, membrane-associated polypeptide 2C and its precursor, 2BC, is believed to be required for initiation and elongation of viral RNA synthesis. Previous studies have shown that the 2C polypeptide specifically interacts with the 3'-terminal sequence of poliovirus negative-strand RNA. This interaction is facilitated by the presence of the conserved sequence UGUUUU in the "stem a" within the 3'-terminal cloverleaf structure, We show that the 2C precursor. 2BC, also interacts with the 3'-terminal cloverleaf of the negative-strand RNA. We also demonstrate here that interaction of 2C/2BC with the negative strand 3'-terminal sequence not only depends on an intact "stem a" containing the UGUUUU sequence but is equally influenced by the presence of an intact "stem b" within the negative-strand cloverleaf, The results presented here suggest that the spatial configuration of stem a UGUUUU sequence with respect to an intact stem-b is crucial for 2C/2BC interaction with the 3'-terminal negative-strand cloverleaf structure. (C) 2001 Academic Press.