An Ex Vivo Tissue Culture Method for Discovering Cell Dynamics Involved in Stromal Vascular Fraction Vasculogenesis Using the Mouse Mesentery.
An Ex Vivo Tissue Culture Method for Discovering Cell Dynamics Involved in Stromal Vascular Fraction Vasculogenesis Using the Mouse Mesentery.
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DOI：
10.1007/978-1-0716-2059-5_12
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发表时间：
2022-01-01
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影响因子：
--
通讯作者：
Murfee, Walter L
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文献类型：
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作者：
Majbour, Dima;Suarez-Martinez, Ariana D;Murfee, Walter L
从脂肪组织中分离的基质血管组分（SVF）被鉴定为由内皮细胞、内皮祖细胞、周细胞、平滑肌细胞、成纤维细胞和免疫细胞组成的丰富的细胞来源。SVF由于其丰富的细胞生态位而代表了用于生长新的血液微血管的有前景的治疗性异种细胞来源。然而，SVF在活组织内的时空动力学在很大程度上仍然未知。本章的目的是描述在小鼠肠系膜组织上培养SVF的方案，以帮助发现SVF动态变化和相关血管随时间的生长。从成年小鼠的腹股沟脂肪中分离SVF并接种到肠系膜组织上。然后将组织培养长达5天，并用内皮细胞和周细胞标记物标记。代表性结果表明观察到SVF衍生的血管发生，其特征在于从头血管形成和随后的血管连接。
Stromal vascular fraction (SVF), isolated from adipose tissue, identifies as a rich cell source comprised of endothelial cells, endothelial progenitor cells, pericytes, smooth muscle cells, fibroblasts, and immune cells. SVF represents a promising therapeutic heterogonous cell source for growing new blood microvessels due to its rich niche of cells. However, the spatiotemporal dynamics of SVF within living tissues remain largely unknown. The objective of this chapter is to describe a protocol for culturing SVF on mouse mesentery tissues in order to aid in the discovery of SVF dynamics and associated vessel growth over time. SVF was isolated from the inguinal adipose from adult mice and seeded onto mesentery tissues. Tissues were then cultured for up to 5days and labeled with endothelial cell and pericyte markers. Representative results demonstrate the observation of SVF-derived vasculogenesis characterized by de novo vessel formation and subsequent vessel connection.