Rapid, microscale, acetyl bromide-based method for high-throughput determination of lignin content in Arabidopsis thaliana
Rapid, microscale, acetyl bromide-based method for high-throughput determination of lignin content in Arabidopsis thaliana
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DOI：
10.1021/jf800775f
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发表时间：
2008-08-27
影响因子：
6.1
通讯作者：
Beatson, Rodger P.
中科院分区：
文献类型：
--
作者：
Chang, Xue Feng;Chandra, Richard;Beatson, Rodger P.
对乙酰溴法进行了改进，使其能够快速、微量地测定拟南芥中木质素的含量，目的是确定植物中控制木质素的基因。改进措施包括减少样品大小、使用微球研磨机、采用改进的快速提取方法、使用冰浴来稳定溶液以及减少溶液体积。该方法具有快速、准确、精密度好的特点，测定结果与乙酰溴化法完全一致。用乙酰溴溶解的拟南芥木质素在280 nm波长处的消光系数为23.35g(-1)L cm(-1)。该值与拟南芥的加入、环境生长条件无关，对木质素结构不敏感。新开发的方法可用于测定拟南芥花序茎中木质素的含量，为木质素相关基因的定位提供依据。
The acetyl bromide method has been modified to enable the rapid microscale determination of lignin content in Arabidopsis with the goal of determining the genes that control lignin in plants. Modifications include reduction in sample size, use of a microball mill, adoption of a modified rapid method of extraction, use of an ice-bath to stabilize solutions and reduction in the volume of solutions. The microscale method was shown to be rapid, accurate and precise with values in agreement with those determined by the full-scale acetyl bromide method. The extinction coefficient for Arabidopsis lignin, dissolved using acetyl bromide, was determined to be 23.35 g(-1) L cm(-1) at 280 nm. This value is independent of the Arabidopsis accession, environmental growth conditions and is insensitive to lignin structure. The newly developed method can be used to determine lignin content in the inflorescence stems of Arabidopsis for mapping of lignin-related genes.