Polyelectrolyte-coated gold nanorods and their interactions with type I collagen.
Polyelectrolyte-coated gold nanorods and their interactions with type I collagen.
复制标题
DOI：
10.1016/j.biomaterials.2009.07.011
复制
发表时间：
2009-10
期刊：
影响因子：
14
通讯作者：
Goldsmith, Edie C.
中科院分区：
文献类型：
--
作者：
Wilson, Christopher G.;Sisco, Patrick N.;Gadala-Maria, Francis A.;Murphy, Catherine J.;Goldsmith, Edie C.
金纳米棒（AuNRs）具有独特的光学性质，可用于许多生物医学应用，但AuNRs与蛋白质，特别是细胞外基质（ECM）之间的相互作用知之甚少。在这里，金核受体对自组装，力学和重塑的I型胶原蛋白凝胶的影响进行了检查在体外。用聚电解质多层（PEM）修饰AuNR以最大限度地减少细胞毒性，并通过浊度测定、流变学测试和显微镜检查具有不同末端聚合物化学成分的AuNR与胶原蛋白的相互作用。凝胶收缩试验用于检查PEM包被的AuNR对细胞介导的胶原重塑的影响。聚阴离子封端的金核受体显着减少胶原蛋白自组装的滞后（成核）阶段，并显着增加聚合凝胶的动态剪切模量，而聚阳离子封端的金核受体对胶原蛋白的机械性能没有影响。聚阴离子和聚阳离子终止的金核受体显着抑制心脏成纤维细胞的胶原凝胶收缩，和纳米粒子被定位在内，外，和细胞外的隔间，这表明PEM包被的金核受体通过多种机制影响细胞行为。这些结果证明了纳米颗粒-ECM相互作用在确定纳米颗粒的生物活性中的重要性。
Gold nanorods (AuNRs) have unique optical properties for numerous biomedical applications, but the interactions between AuNRs and proteins, particularly those of the extracellular matrix (ECM), are poorly understood. Here the effects of AuNRs on the self-assembly, mechanics, and remodeling of type I collagen gels were examined in vitro. AuNRs were modified with polyelectrolyte multilayers (PEMs) to minimize cytotoxicity, and AuNRs with different terminal polymer chemistries were examined for their interactions with collagen by turbidity assays, rheological tests, and microscopy. Gel contraction assays were used to examine the effects of the PEM-coated AuNRs on cell-mediated collagen remodeling. Polyanion-terminated AuNRs significantly reduced the lag (nucleation) phase of collagen self-assembly and significantly increased the dynamic shear modulus of the polymerized gels, whereas polycation-terminated AuNRs had no effect on the mechanical properties of the collagen. Both polyanion- and polycation-terminated AuNRs significantly inhibited collagen gel contraction by cardiac fibroblasts, and the nanoparticles were localized in intra-, peri-, and extracellular compartments, suggesting that PEM-coated AuNRs influence cell behavior via multiple mechanisms. These results demonstrate the significance of nanoparticle-ECM interactions in determining the bioactivity of nanoparticles.
登录
查看更多内容
影响因子：
2.9
作者：
BLUNK, T;HOCHSTRASSER, DF;MULLER, RH
通讯作者：
MULLER, RH
影响因子：
3.9
作者：
Dorris, Annie;Rucareanu, Simona;Lennox, R. Bruce
通讯作者：
Lennox, R. Bruce
影响因子：
4
作者：
Collin, Olivier;Tracqui, Philippe;Planus, Emmanuelle
通讯作者：
Planus, Emmanuelle
DOI：
10.1152/ajpheart.00514.2007
发表时间：
2007-09-01
影响因子：
4.8
作者：
Banerjee, Indroneal;Fuseler, John W.;Baudino, Troy A.
通讯作者：
Baudino, Troy A.
影响因子：
14
作者：
Freeman, Inbar;Kedem, Alon;Cohen, Smadar
通讯作者：
Cohen, Smadar