Enhanced expression of beta-adrenergic receptor kinase 1 in the hearts of cardiomyopathic Syrian hamsters, BIO53.58.
Enhanced expression of beta-adrenergic receptor kinase 1 in the hearts of cardiomyopathic Syrian hamsters, BIO53.58.
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心肌病叙利亚仓鼠心脏中 β-肾上腺素受体激酶 1 的表达增强，BIO53.58。
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
A. Kitabatake
中科院分区：
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作者：
K. Urasawa;I. Yoshida;C. Takagi;H. Onozuka;T. Mikami;H. Kawaguchi;A. Kitabatake
我们通过逆转录和随后的聚合酶链反应从叙利亚仓鼠心脏中克隆了β-肾上腺素能受体激酶1（β ARK 1）cDNA的整个编码序列。克隆的cDNA全长2067个核苷酸，编码689个氨基酸。该序列与大鼠β ARK 1的同源性为95%，与人同源性为90%。心肌病叙利亚仓鼠BIO53.58已被用作充血性心力衰竭的模型动物。M型超声心动图证实20周龄BIO53.58的左心室收缩力明显降低。与对照仓鼠F1 b相比，BIO53.58心脏中β ARK 1 mRNA的表达显著增加，表明增强的β ARK 1表达作为负反馈机制发挥作用，以通过β-肾上腺素能受体的磷酸化维持细胞内稳态，对抗儿茶酚胺的加速刺激。
We cloned an entire encoding sequence of beta-adrenergic receptor kinase 1 (beta ARK1) cDNA from the hearts of Syrian hamsters through reverse transcription and subsequent polymerase chain reaction. The cloned cDNA contained 2067 nucleotides coding 689 amino acids. The sequence had 95% homology to rat beta ARK1 and 90% homology to human homologue. Cardiomyopathic Syrian hamster, BIO53.58, has been used as a model animal of congestive heart failure. M-mode echocardiography confirmed that left ventricular contractility of 20-week-old BIO53.58 was markedly reduced. The expression of beta ARK1 mRNA in the hearts of BIO53.58 was significantly increased compared to control hamsters, F1b, suggesting that the enhanced beta ARK1 expression is acting as a negative feedback mechanism in order to maintain intracellular homeostasis against accelerated stimulation by catecholamines via phosphorylation of beta-adrenergic receptor.