Differential production of Th1- and Th2-type chemokines by mouse Langerhans cells and splenic dendritic cells
Differential production of Th1- and Th2-type chemokines by mouse Langerhans cells and splenic dendritic cells
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DOI：
10.1111/j.0022-202x.2004.23607.x
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发表时间：
2005-02-01
影响因子：
6.5
通讯作者：
Tamaki, K
中科院分区：
文献类型：
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作者：
Fujita, H;Asahina, A;Tamaki, K
一些趋化因子专门吸引辅助性 T 1 (Th1) 细胞，而其他趋化因子则吸引辅助性 T 2 (Th2) 细胞。在本研究中，我们研究了朗格汉斯细胞 (LC) 与脾 CD11c(+) 树突状细胞 (DC) 产生 Th1 和 Th2 型趋化因子的能力。我们使用淘选方法从 BALB/c 小鼠皮肤中制备了高纯度 (>95%) LC。对于 Th1 型趋化因子，外源刺激，例如干扰素-γ (IFN-1)、脂多糖或多聚肌苷-聚胞苷酸，对于在 LC 和脾 DC 中产生大量 CXCL10、CXCL9 和 CXCL11 是必需的。 LC。总体而言，与脾 DC 相比，其产生 Th1 型趋化因子的能力较低。至于Th2型趋化因子，LC。即使没有外源刺激，脾 DC 也能在培养过程中持续产生高水平的 CCL22 和 CCL17，但脾 DC 则不然。 Th2型趋化因子的产生受到复杂的调节。尤其。 LC 上调 IL-4 上调 CCL22 和 CCL17 的产生，而 IFN-γ 下调 CCL22 和 CCL17 的产生。值得注意的是。在任何测试条件下，LC 产生的 Th2 型趋化因子都比脾 DC 多得多。最后，使用趋化因子受体转染的 2B4 T 细胞证明 LC 产生的 Th1 型和 Th2 型趋化因子具有功能。在没有 IFN-γ 的情况下，LC 大量产生 CC 趋化因子受体 4 配体，这可能是区分 LC 与其他 DC 的重要特征。
Some chemokines specifically attract T helper 1 (Th1) cells, whereas others attract T helper 2 (Th2) cells. In this study, we investigated the capacity of Langerhans cells (LC) to produce Th1- and Th2-type chemokines in comparison with that of splenic CD11c(+) dendritic cells (DC). We prepared highly purified (>95%) LC from BALB/c mouse skin using the panning method. With regard to Th1-type chemokines, exogenous stimulus, such as interferon-gamma (IFN-1), lipopolysaccharide, or polyinosinic-polycytidylic acid, was mandatory for the production of substantial amounts of CXCL10, CXCL9, and CXCL11 both in LC and splenic DC. LC. as a whole.. exhibited low ability to produce Th1-type chemokines in comparison with splenic DC. As for Th2-type chemokines, LC. but not splenic DC, produced high levels of CCL22 and CCL17 constitutively during culture even without exogenous stimuli. The production of Th2-type chemokines was regulated in a complicated manner. In particular. interleukin-4 upregulated, and IFN-gamma downregulated both CCL22 and CCL17 production by LC. Of note. LC produced much more amounts of Th2-type chemokines than splenic DC under any conditions tested. Finally, Th1- and Th2-typ e chemokines produced by LC were shown to be functional using chemokine receptor-transfected-2B4 T cells. The high production of CC chemokine receptor 4 ligands by LC in the absence of IFN-gamma may be an important character discriminating LC from other DC.