Growing reproducible biofilms with respect to structure and viable cell counts
Growing reproducible biofilms with respect to structure and viable cell counts
复制标题
DOI：
10.1016/s0167-7012(01)00280-9
复制
发表时间：
2001-10-01
影响因子：
2.2
通讯作者：
Lewandowski, Z
中科院分区：
文献类型：
--
作者：
Jackson, G;Beyenal, H;Lewandowski, Z
我们已经开发了一种新的方法，生长4天的生物膜是可再生的，相对于活细胞数量和生物膜结构。为了证明该方法的实用性，我们在平板反应器中在明确定义的条件下生长由铜绿假单胞菌（ATCC #700829）、荧光假单胞菌（ATCC #700830）和肺炎克雷伯氏菌（ATCC #700831）组成的生物膜18次：流速、营养物浓度、温度、接种物和生长速率。得到的4天龄的生物膜约为200-300 μ m厚，并表现出高度的再现性。每单位表面积积累的活细胞的数量和生物膜的真实孔隙率在10%误差内重现。我们还量化了其他参数表征生物膜结构，使用生物膜成像技术：分形维数，纹理熵和扩散距离作为辅助参数表征生物膜积累的再现性，作为分析的结果，我们引入了一个新的参数，以更好地量化和表征生物膜中的活细胞的数量，“特定的活细胞数”（SN-VC）。该参数是相对于被生物膜覆盖的表面积和相对于生物膜的生物量标准化的活细胞数。这个新的描述符代表生物膜积累的动态比传统上使用的菌落形成单位（CFU）的生物膜覆盖的每表面积。因为它不仅考虑了表面覆盖率，而且考虑了生物膜厚度。(C)2001 Elsevier Science B. V.保留所有权利。
We have developed a new method of growing 4-day-old biofilms that are reproducible, with respect to viable cell number and biofilm structure. To demonstrate the utility of the method, we grew biofilms composed of Pseudomonas aeruginosa (ATCC#700829), P. fluorescens (ATCC#700830) and Klebsiella pneumoniae (ATCC#700831), 18 times in flat-plate reactors under well-defined conditions of: flow rate, nutrient concentration, temperature, inoculum and growth rate. The resulting 4-day-old biofilms were approximately 200-300 mum thick and exhibited a high degree of reproducibility. The number of viable cells that accumulated per unit surface area and the biofilm a-real porosity were reproduced within 10% error. We have also quantified other parameters characterizing biofilm structure using biofilm-imaging techniques: fractal dimension, textural entropy and diffusion distance as auxiliary parameters characterizing the reproducibility of biofilm accumulation, As a result of analysis, we have introduced a new parameter to better quantify and characterize the number of viable cells in biofilms, "specific number of viable cells" (SN-VC). This parameter is the viable cell number normalized with respect to the surface area covered by the biofilm and with respect to the biomass of the biofilm. This new descriptor represents the dynamics of biofilm accumulation better than the traditionally used colony-forming unit (CFU) per surface area covered by the biofilm. because it accounts not only for the surface coverage but also for the biofilm thickness. (C) 2001 Elsevier Science B.V. All rights reserved.