Ectopic expression of OsYAB1 causes extra stamens and carpels in rice
Ectopic expression of OsYAB1 causes extra stamens and carpels in rice
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DOI：
10.1007/s11103-004-2648-y
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发表时间：
2004-09-01
影响因子：
5.1
通讯作者：
An, G
中科院分区：
文献类型：
--
作者：
Jang, S;Hur, J;An, G
YABBY 基因蛋白家族的成员是植物特异性转录因子，在决定器官极性方面发挥着关键作用。我们从水稻中分离出了编码 YABBY 蛋白的 cDNA 克隆。这种蛋白质 OsYAB1 与拟南芥 YAB2 (50.3%) 和 YAB5 (47.6%) 相似。它带有一个锌指基序和一个 YABBY 结构域，就像拟南芥中的那样。 OsYAB1 和 GFP 之间的融合蛋白位于细胞核中。 RNA凝胶印迹分析表明OsYAB1基因优先在花中表达。原位杂交实验还表明转录本在雄蕊和心皮原基中积累。然而，与拟南芥 YABBY 基因不同，OsYAB1 基因在组织中不显示极性表达模式。口腔器官。我们异位表达 OsYAB1 的转基因植物在营养生长期是正常的，但随后表现出异常。口腔结构。与野生型相比，小穗含有多余的雄蕊和心皮。这些结果表明 OsYAB1 在分生组织发育以及雄蕊和心皮的维持中发挥主要作用，而不是在确定极性中发挥主要作用。
Members in the YABBY gene family of proteins are plant-specific transcription factors that play critical roles in determining organ polarity. We have isolated a cDNA clone from rice that encodes a YABBY protein. This protein, OsYAB1, is similar to Arabidopsis YAB2 (50.3%) and YAB5 (47.6%). It carries a zinc-finger motif and a YABBY domain, as do those in Arabidopsis. A fusion protein between OsYAB1 and GFP is located in the nucleus. RNA gel-blot analysis showed that the OsYAB1 gene is preferentially expressed in flowers. In-situ hybridization experiments also indicated that the transcript accumulated in the stamen and carpel primordia. Unlike the Arabidopsis YABBY genes, however, the OsYAB1 gene does not show polar expression pattern in the tissues of. oral organs. Our transgenic plants that ectopically expressed OsYAB1 were normal during the vegetative growth period, but then showed abnormalities in their. oral structures. Spikelets contained supernumerary stamens and carpels compared with those of the wild types. These results suggest that OsYAB1 plays a major role in meristem development and maintenance of stamens and carpels, rather than in determining polarity.