Fe(III)-enhanced Azo Reduction by Shewanella decolorationis S12
Fe(III)-enhanced Azo Reduction by Shewanella decolorationis S12
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DOI：
10.1007/s00253-006-0773-z
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发表时间：
2007-01
影响因子：
5
通讯作者：
Meiying Xu;Jun Guo;Xiang-yi Kong;Xingjuan Chen;G. Sun
中科院分区：
文献类型：
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作者：
Meiying Xu;Jun Guo;Xiang-yi Kong;Xingjuan Chen;G. Sun
脱色希瓦氏菌S12具有较高的偶氮染料脱色率和异化还原Fe(III)能力。在厌氧条件下，当S12菌体中Fe(III)和偶氮染料共存时，异化Fe(III)还原和偶氮染料生物脱色同时发生。此外，Fe(III)的存在也促进了染料的脱色。当添加1 mM Fe(III)时，培养3h后，甲基红的脱色率为72.1%，而在无Fe(III)的条件下，甲基红的脱色率仅为60.5%。当Fe(III)浓度从0增加到6 mM时，脱色效率增加。介导染料脱色和Fe(III)还原的酶活性不受细胞对Fe(III)和偶氮染料的预适应的影响，也不受氯霉素的影响。Fe(III)还原酶和偶氮还原酶均为膜结合酶。呼吸电子传递链抑制剂美他隆、敌草酚和豆蔻素对Fe(III)的还原作用明显不同于对偶氮染料的脱色作用。
Shewanella decolorationis S12 is capable of high rates of azo dye decolorization and dissimilatory Fe(III) reduction. Under anaerobic conditions, when Fe(III) and azo dye were copresent in S12 cultures, dissimilatory Fe(III) reduction and azo dye biodecolorization occurred simultaneously. Furthermore, the dye decolorization was enhanced by the presence of Fe(III). When 1 mM Fe(III) was added, the methyl red decolorizing efficiency was 72.1% after cultivation for 3 h, whereas the decolorizing efficiency was only 60.5% in Fe(III)-free medium. The decolorizing efficiencies increased as the concentration of Fe(III) was increased from 0 to 6 mM. Enzyme activities, which mediate the dye decolorization and Fe(III) reduction, were not affected by preadaption of cells to Fe(III) and azo dye nor by the addition of chloramphenicol. Both the Fe(III) reductase and the azo reductase were membrane associated. The respiratory electron transport chain inhibitors metyrapone, dicumarol, and stigmatellin showed significantly different effects on Fe(III) reduction than on azo dye decolorization.