Streptococcus cristatus attenuates Fusobacterium nucleatum-induced interleukin-8 expression in oral epithelial cells.
Streptococcus cristatus attenuates Fusobacterium nucleatum-induced interleukin-8 expression in oral epithelial cells.
复制标题
冠状链球菌减弱具核梭杆菌诱导的口腔上皮细胞中白细胞介素 8 的表达。
DOI：
10.1111/j.1600-0765.2007.01057.x
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发表时间：
2008
影响因子：
3.5
通讯作者：
Rudney,JD
中科院分区：
文献类型：
--
作者：
Zhang,G;Chen,R;Rudney,JD
背景和目的：口腔上皮细胞可能受到细胞内多微生物菌群的侵袭，包括病原体和共生菌。多种口腔病原体可诱导口腔上皮细胞产生白细胞介素- 8，这是一种有效的中性粒细胞趋化剂。来自肠道的证据表明，共生物种可能调节对病原体的炎症反应。本研究的目的是检测口腔上皮细胞对口腔促炎物种——核梭杆菌（Fusobacterium nucleatum）和口腔共生体——cristatus链球菌（Streptococcus cristatus）的白细胞介素- 8反应。材料和方法：KB、TERT‐2、TR146和SCC15细胞与f . nucleatumds共培养。在37°C、5% co2和各种条件下，单独或组合cristatus。用逆转录聚合酶链反应分析白细胞介素- 8 mRNA的表达，酶联免疫吸附法检测白细胞介素- 8蛋白的分泌。nucleatumalone引起了强有力的白细胞介素- 8反应。cristatus在口腔上皮细胞中没有显著的白细胞介素- 8表达。当出现在一起时，S。cristatusattenuated theF。在四种口腔上皮细胞系中不同程度地诱导白细胞介素- 8的产生。s。冠态不依赖于其活力和与f的共聚集。与可溶性细菌产物无关，似乎需要细菌与上皮细胞接触。在其他几种口腔链球菌中也发现了类似的效果。结论：我们的数据表明是这样。鸡冠可能对口腔上皮细胞对核攻击的白细胞介素- 8反应发挥免疫调节作用。
Background and Objective:Oral epithelial cells may be invaded by a polymicrobial intracellular flora, including pathogens together with commensals. Various oral pathogens can induce the production of interleukin‐8, a potent neutrophil chemotractant, in oral epithelial cells. Evidence from the gut suggests that commensal species may modulate inflammatory responses to pathogens. The aim of this study was to examine the interleukin‐8 responses of oral epithelial cells to an oral pro‐inflammatory species,Fusobacterium nucleatum, in combination with an oral commensal,Streptococcus cristatus.Material and methods:KB, TERT‐2, TR146 and SCC15 cells were cocultured withF. nucleatumandS. cristatus, either alone or in combination, at 37°C in 5% CO2under various conditions. The mRNA expression of interleukin‐8 was analyzed by reverse transcription–polymerase chain reaction and protein secretion was measured by enzyme‐linked immunosorbent assay.Results:F. nucleatumalone evoked a potent interleukin‐8 response, whereasS. cristatusalone did not induce significant interleukin‐8 expression in oral epithelial cells. When present together,S. cristatusattenuated theF. nucleatum‐induced interleukin‐8 production in the four oral epithelial cell lines to varying degrees. The inhibitory effect ofS. cristatuswas independent of its viability and its co‐aggregation withF. nucleatum, was not related to soluble bacterial products and appeared to require bacterial contact with epithelial cells. Similar effects were seen with several other species of oral streptococci.Conclusion:Our data suggest thatS. cristatusmay exert immunomodulatory effects on the interleukin‐8 response of oral epithelial cells toF. nucleatumchallenge.