Metabolomic profiling of reactive persulfides and polysulfides in the aqueous and vitreous humors.
Metabolomic profiling of reactive persulfides and polysulfides in the aqueous and vitreous humors.
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DOI：
10.1038/srep41984
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发表时间：
2017-02-07
影响因子：
4.6
通讯作者：
Nakazawa T
中科院分区：
文献类型：
--
作者：
Kunikata H;Ida T;Sato K;Aizawa N;Sawa T;Tawarayama H;Murayama N;Fujii S;Akaike T;Nakazawa T
我们研究了在水和玻璃体液中的活性过硫化物和多硫化物的代谢谱。对18例18眼糖尿病合并糖尿病视网膜病变患者行微切口玻璃体切割联合白内障手术。收集房水和玻璃体液样本，并对反应性过硫化物和多硫化物（多硫化物组）进行基于质谱的代谢组学分析。还用Alcohol Blue测定法评价了反应性多硫化物物质对氧化应激（用H2 O2诱导）下永生化视网膜细胞（RGC-5细胞系）活力的影响。实验表明，半胱氨酸过硫化物（CysSSH），氧化型谷胱甘肽三硫化物（GSSSG）和胱氨酸在房水中升高，CysSSH，Cys和胱氨酸在玻璃体中升高。此外，GSSSG，胱氨酸和CysSSH水平相关的水和玻璃体液。比较，在DM和对照组，血浆水平的反应性过硫化物和多硫化物显示，他们没有什么不同。体外研究结果表明，活性多硫化物物种增加氧化应激下的细胞活力。因此，各种反应性过硫化物和多硫化物似乎存在于眼中，并且一些对氧化应激具有保护作用的反应性硫化物物质在DM眼的房水和玻璃体液中上调。
We investigate the metabolomic profile of reactive persulfides and polysulfides in the aqueous and vitreous humors. Eighteen eyes of 18 consecutive patients with diabetes mellitus (DM) and diabetic retinopathy underwent microincision vitrectomy combined with cataract surgery. Samples of the aqueous and vitreous humors were collected and underwent mass spectrometry-based metabolomic profiling of reactive persulfides and polysulfides (polysulfidomics). The effect of reactive polysulfide species on the viability of immortalized retinal cells (the RGC-5 cell line) under oxidative stress (induced with H2O2) was also evaluated with an Alamar Blue assay. The experiments showed that cysteine persulfides (CysSSH), oxidized glutathione trisulfide (GSSSG) and cystine were elevated in the aqueous humor, and CysSSH, Cys, and cystine were elevated in the vitreous. Furthermore, GSSSG, cystine, and CysSSH levels were correlated in the aqueous and vitreous humors. A comparison, in DM and control subjects, of plasma levels of reactive persulfides and polysulfides showed that they did not differ. In vitro findings revealed that reactive polysulfide species increased cell viability under oxidative stress. Thus, various reactive persulfides and polysulfides appear to be present in the eye, and some reactive sulfide species, which have a protective effect against oxidative stress, are upregulated in the aqueous and vitreous humors of DM eyes.