Thylakoid Membrane Maturation and PSII Activation Are Linked in Greening Synechocystis sp PCC 6803 Cells
Thylakoid Membrane Maturation and PSII Activation Are Linked in Greening Synechocystis sp PCC 6803 Cells
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DOI：
10.1104/pp.113.224428
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发表时间：
2013-10-01
期刊：
影响因子：
7.4
通讯作者：
Schneider, Dirk
中科院分区：
文献类型：
--
作者：
Barthel, Sandra;Bernat, Gabor;Schneider, Dirk
类囊体膜是叶绿体和蓝藻的典型和基本特征。虽然它们是至关重要的光合生长的蓝藻细胞，类囊体膜的生物起源还没有得到很好的理解。暗生长的集胞藻PCC 6803细胞只含有基本的类囊体膜，但仍然有相对较高的量的藻胆体，非活性光系统II和活性光系统I中心。在将黑暗生长的集胞藻属PCC 6803细胞转移到光照下后，监测集胞藻属PCC 6803细胞的“绿化”，即类囊体膜形成和光合电子传递反应的恢复。典型的类囊体膜系统的完全恢复后，在24小时内观察到6至8小时的初始滞后期。此外，光系统II复合物的激活和功能性光合电子传递链的恢复似乎与有组织的类囊体膜对的生物发生有关。
Thylakoid membranes are typical and essential features of both chloroplasts and cyanobacteria. While they are crucial for phototrophic growth of cyanobacterial cells, biogenesis of thylakoid membranes is not well understood yet. Dark-grown Synechocystis sp. PCC 6803 cells contain only rudimentary thylakoid membranes but still a relatively high amount of phycobilisomes, inactive photosystem II and active photosystem I centers. After shifting dark-grown Synechocystis sp. PCC 6803 cells into the light, "greening" of Synechocystis sp. PCC 6803 cells, i.e. thylakoid membrane formation and recovery of photosynthetic electron transport reactions, was monitored. Complete restoration of a typical thylakoid membrane system was observed within 24 hours after an initial lag phase of 6 to 8 hours. Furthermore, activation of photosystem II complexes and restoration of a functional photosynthetic electron transport chain appears to be linked to the biogenesis of organized thylakoid membrane pairs.