Melanocyte-stimulating hormone facilitates hypermelanosis on the non-eyed side of the barfin flounder, a pleuronectiform fish
Melanocyte-stimulating hormone facilitates hypermelanosis on the non-eyed side of the barfin flounder, a pleuronectiform fish
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DOI：
10.1016/j.aquaculture.2007.05.037
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发表时间：
2007-09
期刊：
影响因子：
4.5
通讯作者：
Takeshi Yamanome;H. Chiba;A. Takahashi
中科院分区：
文献类型：
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作者：
Takeshi Yamanome;H. Chiba;A. Takahashi
巴鳍比目鱼(Verasper Moseri)是一种胸鳍形目鱼类，具有很高的商业价值，在日本北部的水产养殖和资源开发方面具有很好的前景。在培养条件下经常发生的无眼侧黑色素沉着症降低了它的商业价值。黑色素浓缩激素(MCH)和黑素细胞刺激素(MSH)这两种具有相反作用的多肽激素与鱼类的颜色变化有关。我们之前已经报道了MCH在预防黑色素沉着方面的积极作用。在这里，我们研究了MSH对黑色素沉着症发生的影响。单次注射Des-Ac-α-msh[0.01nmoL/g-10nmoL/g(0.016μg-16μg/g)]不改变眼侧体色，单次注射Mch[0.1nmoL/g(0.21nmol/g)μg/g]使眼侧颜色变浅。同时注射DES-Ac-α-MSH(0.01nmol/g-10nmol/g)的鱼与注射高氯酸乙酯(0.1nmoL/g)和同时注射DES-Ac-MsH(0.01nmoL/g-10nmoL/g)的鱼相比，眼侧亮度无明显差异。这些结果表明，MSH不能抑制MCH在体表的褪色效应。另一方面，与对照鱼相比，植入含有DES-Ac-α-msh的胆固醇颗粒(280gμg，每29天两次)会增加巴鳍比目鱼无眼一侧的黑色素沉着。MSH处理的鱼的眼侧身体比对照鱼更暗；因此，MSH参与了非眼侧皮肤的形态颜色变化，包括异位黑色素的合成。
A pleuronectiform fish, the barfin flounder, Verasper moseri, is promising for aquaculture and resource enhancement in Northern Japan due to its high commercial value. Hypermelanosis of its non-eyed side, which frequently occurs under culture conditions, diminishes its commercial value. Two peptide hormones, melanin-concentrating hormone (MCH) and melanocyte-stimulating hormone (MSH), having opposing actions, are associated with the color changes of fish. We have previously reported the positive effect of MCH in preventing hypermelanosis. Here, we examined the effects of MSH on the occurrence of hypermelanosis. A single injection of Des-Ac-α-MSH [0.01 nmol/g–10 nmol/g (0.016 μg–16 μg/g)] did not change the eyed-side body color, while a single injection of MCH [0.1 nmol/g (0.21 μg/g)] made the eyed-side color paler. No difference was observed in eyed-side lightness between fish injected with MCH (0.1 nmol/g) and those receiving MCH (0.1 nmol/g) and an increased amount of Des-Ac-α-MSH (0.01 nmol/g–10 nmol/g) simultaneously. These results indicate that MSH does not suppress the in vivo body color-paling effects of MCH in barfin flounders. On the other hand, implantation of a cholesterol pellet containing Des-Ac-α-MSH (280 μg, twice at 29-day interval) increased hypermelanosis of the non-eyed side of barfin flounders compared to control fish. Eyed-side bodies of MSH-treated fish were darker than control fish; thus, MSH is involved in morphological color change including ectopic melanin synthesis in non-eyed-side skin.