Asymmetric GUVs Prepared by MβCD-Mediated Lipid Exchange: An FCS Study
Asymmetric GUVs Prepared by MβCD-Mediated Lipid Exchange: An FCS Study
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DOI：
10.1016/j.bpj.2010.11.051
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发表时间：
2011-01-05
影响因子：
3.4
通讯作者：
London, Erwin
中科院分区：
文献类型：
--
作者：
Chiantia, Salvatore;Schwille, Petra;London, Erwin
我们报道了一种获得稳定的不对称巨单层囊泡（GUV）的简单方法。荧光相关光谱被用来定量表征囊泡的性质。脑鞘磷脂（bSM）交换成二油酰磷脂酰胆碱（DOPC）GUV后，含bSM的外叶的侧向扩散减少，而含DOPC的内叶的侧向扩散基本不变，证实了不对称性和内外叶物理状态之间缺乏耦合。相反，bSM交换到脑磷脂酰胆碱囊泡后，两个小叶的侧向扩散减少。因此，不对称的GUV应该是有用的研究背后的分子机制interleaflet耦合。
We report a simple method to obtain stable asymmetric giant unilamellar vesicles (GUVs). Fluorescence correlation spectroscopy was used to quantitatively characterize vesicle properties. After brain sphingomyelin (bSM) was exchanged into dioleoylphosphatidylcholine (DOPC) GUVs, lateral diffusion in the bSM-containing outer leaflet decreased, whereas that in the DOPC-containing inner leaflet was largely unchanged, confirming asymmetry and a lack of coupling between the physical states of the inner and outer leaflets. In contrast, after bSM was exchanged into brain phosphatidylcholine vesicles, lateral diffusion decreased in both leaflets. Thus, asymmetric GUVs should be useful for investigating the molecular mechanisms behind interleaflet coupling.