Hair follicle differentiation: expression, structure and evolutionary conservation of the hair type II keratin intermediate filament gene family.
Hair follicle differentiation: expression, structure and evolutionary conservation of the hair type II keratin intermediate filament gene family.
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发表时间：
1992-02
期刊：
影响因子：
4.6
通讯作者：
B. Powell;L. Crocker;G. Rogers
中科院分区：
文献类型：
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作者：
B. Powell;L. Crocker;G. Rogers
在毛囊发育过程中，几个细胞流被编程为从毛囊球的细胞群分化。在毛细胞中，许多角蛋白基因家族被转录激活。我们描述了II型角蛋白中间丝（IF）基因家族的特征，该基因家族在卵泡分化早期表达。在绵羊毛中，表达四种II型IF蛋白。一个基因已经完全测序，三个基因的表达进行了详细检查。测序的基因编码一个55 × 10（3）Mr的II型角蛋白IF蛋白质家族，命名为KII-9的新命名法，我们已经采用和介绍。该基因具有与表皮II型角蛋白IF基因相似的外显子/内含子结构。原位杂交实验表明，这些基因在毛皮质细胞中表达，而在外根鞘、内根鞘和髓质细胞中不表达。在毛发角质形成细胞分化过程中，II型IF基因依次被激活并在相同的细胞中共表达。首先在位于真皮乳头附近的毛囊球中间的细胞中检测到表达，随后，两个基因在分化中的角质形成细胞中转录激活，因为它们向上迁移，在球的上部。第四个II型IF基因随后被激活。具有相同表达模式的基因在序列上也密切相关，并且在这些基因的启动子中存在许多保守元件，包括在共表达的I型IF基因和三个其它毛发角蛋白基因的启动子中也发现的新元件。
During hair follicle development several cell streams are programmed to differentiate from the cell population of the follicle bulb. In the hair cells, a number of keratin gene families are transcriptionally activated. We describe the characterization of the type II keratin intermediate filament (IF) gene family which is expressed early in follicle differentiation. In sheep wool, four type II IF proteins are expressed. One gene has been completely sequenced and the expression of three of the genes examined in detail. The sequenced gene encodes a 55 x 10(3) Mr protein of the type II keratin IF protein family, designated KII-9 in the new nomenclature we have adopted and described in the Introduction. The gene has a similar exon/intron structure to the epidermal type II keratin IF genes. In situ hybridization experiments show that the genes are expressed in the hair cortical cells but not in the cells of the outer root sheath, inner root sheath or medulla. During hair keratinocyte differentiation the type II IF genes are sequentially activated and coexpressed in the same cells. Expression is first detected in cells in the middle of the follicle bulb located near the dermal papilla and, subsequently, two of the genes are transcriptionally activated in the differentiating keratinocytes as they migrate upwards, in the upper part of the bulb. A fourth type II IF gene is activated later. The genes with the same expression pattern are also closely related in sequence and a number of conserved elements are present in the promoters of those genes, including a novel element which is also found in the promoter of a coexpressed type I IF gene and three other hair keratin genes.