Interleukin-6 stimulates neutrophil production of platelet-activating factor
Interleukin-6 stimulates neutrophil production of platelet-activating factor
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DOI：
10.1002/jlb.59.4.569
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发表时间：
1996-04-01
影响因子：
5.5
通讯作者：
Peterson, VM
中科院分区：
文献类型：
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作者：
Biffl, WL;Moore, EE;Peterson, VM
白细胞介素-6 （IL-6）是损伤和感染急性期反应的整体介质；夸大的IL-6反应与不良临床事件有关。IL-6的确切作用尚不清楚，但它似乎能够调节成熟中性粒细胞（pmn）的功能库。我们之前的研究表明，il -6刺激的pmn比未刺激的pmn被较低浓度的血小板活化因子（PAF）激活。最近，我们发现IL-6通过类似paf的机制抑制PMN凋亡。我们假设IL-6刺激PMNs产生PAF。从健康人供体中分离的PMNs与IL-6 （0.1-100 ng/ml）在37℃下孵育，用薄层色谱法测量脂质产生，用闪烁接近法定量PAF。IL-6（1和10 ng/ml）刺激PMNs产生更多的PAF。PAF的产生与PMN胞质钙的增加有关。这些数据可能为IL-6调节pmn介导的细胞毒性以及PAF在介导IL-6对pmn作用中的作用提供机制见解。
Interleukin-6 (IL-6) is an integral mediator of the acute phase response to injnry and infection; an exaggerated IL-6 response has been associated with adverse clinical events. The precise role of IL-6 is unclear, but it appears capable of modulating the functional repertoire of mature neutrophils (PMNs). Our previous work demonstrated that IL-6-stimulated PMNs are primed by lower concentrations of platelet-activating factor (PAF) than nonstimulated PMNs. Recently, we have found that IL-6 suppresses PMN apoptosis via a PAF-like mechanism. We hypothesized that IL-6 stimulates PMNs to produce PAF. PMNs isolated from healthy human donors were incubated with IL-6 (0.1-100 ng/ml) at 37 degrees C. Lipid production was measured by use of thin-layer chromatography, and PAF quantitated with a scintillation proximity assay. IL-6 (1 and 10 ng/ml) stimulated PMNs to produce increased quantities of PAF. PAF production was associated with an increase in PMN cytosolic calcium. These data may provide mechanistic insight into IL-6 regulation of PMN-mediated cytotoxicity and the role of PAF in mediating IL-6 effects on PMNs.