Reversed-phase high-performance liquid chromatographic screening method for serum steroids using retention index and diode-array detection
Reversed-phase high-performance liquid chromatographic screening method for serum steroids using retention index and diode-array detection
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DOI：
10.1016/s0378-4347(98)00351-x
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发表时间：
1998-11-06
期刊：
影响因子：
--
通讯作者：
Laitalainen, T
中科院分区：
文献类型：
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作者：
Kuronen, P;Volin, P;Laitalainen, T
以1-[4-（2，3-二羟基丙氧基）苯基]-1-烷酮为保留指数标准品，采用反相高效液相色谱乙腈-水梯度洗脱，二极管阵列检测器在线记录紫外吸收光谱，建立了一种多甾体化合物的筛选方法。研究了色谱条件对甾体保留指数的影响。该方法已初步应用于血清样品中类固醇的分析。(C)1998 Elsevier Science B. V.保留所有权利。
A multisteroid screening method has been developed based on the use of 1-[4-(2,3-dihydroxypropoxy)phenyl]-1-alkanones as retention index standards and UV absorbance spectra recorded on-line with a diode-array detector using reversed-phase high-performance liquid chromatographic gradient elution with acetonitrile and water. The effect of chromatographic conditions on retention indices of steroids were studied. The method was tentatively applied to profiling of steroids in serum samples. (C) 1998 Elsevier Science B.V. All rights reserved.