ROLE OF MICROTUBULES IN RANDOM CELL-MIGRATION - STABILIZATION OF CELL POLARITY
ROLE OF MICROTUBULES IN RANDOM CELL-MIGRATION - STABILIZATION OF CELL POLARITY
复制标题
DOI：
10.1002/cm.970270110
复制
发表时间：
1994-01-01
影响因子：
--
通讯作者：
DANIELE, RP
中科院分区：
文献类型：
--
作者：
GLASGOW, JE;DANIELE, RP
用延时视频显微镜观察秋水仙胺破坏微管前后豚鼠肺泡巨噬细胞的形态和运动行为，研究微管在细胞随机迁移中的作用。细胞迁移定量的方向持续时间和速度。运动也与形态极性：细胞具有单一的lamelipodal区域（单极细胞）迁移，而那些缺乏一个lamellipodal（非极性细胞）或与相对lamelipodal区域（双极细胞）没有迁移。在2小时内，秋水仙胺引起极性从80%的单极细胞转变为40%的单极和40%的双极细胞，相应地，迁移细胞的比例从80%下降到40%。对于那些在秋水仙胺存在下继续迁移的细胞（仍为单极），平均持续时间和速度仅略有下降（约20%）。许多单个细胞的持续时间和速度实际上增加了，这表明随机迁移不需要完整的微管。我们的结论是秋水仙胺治疗不稳定的垄断，导致逐渐丧失的垄断性和随之而来的抑制迁移。虽然细胞保持单极，但即使在没有完整微管的情况下，它也会继续迁移，但微管是长期维持细胞单极性所必需的，因此也是持续运动所必需的。(C)1994 Wiley-Liss，Inc.
The role of microtubules in random cell migration was investigated using time lapse videomicroscopy to record in vitro the shape and motile behavior of guinea pig alveolar macrophages before and after disrupting microtubules with colcemid. Cell migration was quantified in terms of directional persistence time and speed. Motility was also correlated with morphological polarity: cells having a single lamellipodal region (monopolar cells) migrated, whereas those lacking a lamellipod (apolar cells) or with opposing lamellipodal regions (bipolar cells) did not migrate. Within 2 hours, colcemid caused a shift in polarity from 80% monopoIar cells to 40% monopolar and 40% bipolar cells and a corresponding decrease from 80% to 40% in the fraction of migrating cells. Mean persistence time and speed decreased only slightly (approximately 20%) for those cells (still monopolar) which continued to migrate in the presence of colcemid. Persistence time and speed actually increased for many individual cells, indicating that random migration did not require intact microtubules. We conclude that colcemid treatment destabilizes monopolarity, leading to the gradual loss of monopolarity and consequent inhibition of migration. While a cell remains monopolar, it will continue to migrate even in the absence of intact microtubules, but microtubules are required for the long-term maintenance of cellular monopolarity and, thus, for continued motility. (C) 1994 Wiley-Liss, Inc.