Nicotinic excitatory postsynaptic potentials in hippocampal CA1 interneurons are predominantly mediated by nicotinic receptors that contain α4 and β2 subunits.
Nicotinic excitatory postsynaptic potentials in hippocampal CA1 interneurons are predominantly mediated by nicotinic receptors that contain α4 and β2 subunits.
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DOI：
10.1016/j.neuropharm.2011.08.024
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发表时间：
2011-12
影响因子：
4.7
通讯作者：
McQuiston, A. Rory
中科院分区：
文献类型：
--
作者：
Bell, Karen A.;Shim, Hoon;Chen, Ching-Kang;McQuiston, A. Rory
关键词：
在海马中，包括α4和β2亚基（α4β2*）在内的烟碱受体的激活促进了记忆的形成。α4β2*受体也可能在尼古丁戒断中发挥作用，其缺失可能导致衰老和阿尔茨海默病（AD）的认知能力下降。然而，人们对它们在海马体中的细胞功能知之甚少。因此，我们利用光遗传学、全细胞膜片箝位和电压敏感染料（VSD）成像技术，测量了海马CA1的尼古丁兴奋性突触后电位（EPSPs）。在抑制性中间神经元亚群中，ACh的释放导致α4β2*烟碱受体激活介导的缓慢去极化（上升时间常数33.2±6.5 ms，衰减时间常数138.6±27.2 ms）。这些中间神经元的躯体和树突位于取向层（SO）和空隙层-分子层（SLM）。此外，α4β2*烟碱性epsp在SLM中最多。因此，我们的数据表明，海马CA1间神经元中的烟碱性epsp主要由α4β2*烟碱受体介导，它们的激活可能优先影响海马CA1 SLM的海外输入。
In the hippocampus, activation of nicotinic receptors that include α4 and β2 subunits (α4β2*) facilitates memory formation. α4β2* receptors may also play a role in nicotine withdrawal, and their loss may contribute to cognitive decline in aging and Alzheimer’s disease (AD). However, little is known about their cellular function in the hippocampus. Therefore, using optogenetics, whole cell patch clamping and voltage-sensitive dye (VSD) imaging, we measured nicotinic excitatory postsynaptic potentials (EPSPs) in hippocampal CA1. In a subpopulation of inhibitory interneurons, release of ACh resulted in slow depolarizations(rise time constant 33.2 ± 6.5 ms, decay time constant 138.6 ± 27.2 ms)mediated by the activation of α4β2* nicotinic receptors. These interneurons had somata and dendrites located in the stratum oriens(SO) and stratum lacunosum-moleculare (SLM). Furthermore, α4β2* nicotinic EPSPs were largest in the SLM. Thus, our data suggest that nicotinic EPSPs in hippocampal CA1 interneuronsare predominantly mediated by α4β2* nicotinic receptors and their activation may preferentially affect extrahippocampal inputs in SLM of hippocampal CA1.
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影响因子：
3.4
作者：
Jia, Yousheng;Yamazaki, Yoshihiko;Sumikawa, Katumi
通讯作者：
Sumikawa, Katumi
影响因子：
64.8
作者：
Gray, R;Rajan, AS;Dani, JA
通讯作者：
Dani, JA
DOI：
10.1002/neu.20106
发表时间：
2005-03-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Gahring, LC;Persiyanov, K;Rogers, SW
通讯作者：
Rogers, SW
影响因子：
2.5
作者：
Gahring, LC;Persiyanov, K;Rogers, SW
通讯作者：
Rogers, SW
影响因子：
5.5
作者：
Jones, S;Yakel, JL
通讯作者：
Yakel, JL