Pectin and high-amylose maize starch increase caecal hydrogen production and relieve hepatic ischaemia-reperfusion injury in rats
Pectin and high-amylose maize starch increase caecal hydrogen production and relieve hepatic ischaemia-reperfusion injury in rats
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DOI：
10.1017/s0007114511003229
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发表时间：
2012-02-01
影响因子：
3.6
通讯作者：
Kiriyama, Shuhachi
中科院分区：
文献类型：
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作者：
Nishimura, Naomichi;Tanabe, Hiroki;Kiriyama, Shuhachi
我们研究了高H-2产生膳食纤维和抗性淀粉（RS）的喂养是否可以抑制肝脏缺血再灌注（IR）损伤，这是由氧化应激引起的，在大鼠中喂养果胶（Pec）或高直链淀粉玉米淀粉（HAS）饮食。雄性Sprague-Dawley大鼠喂食对照（C）饮食，补充或不补充Pec（0- 5%Pec）或HAS（0- 30%HAS）7天。门静脉H-2浓度随Pec或HAS补充量的增加而呈显著的剂量依赖性增加。血浆丙氨酸和天冬氨酸转氨酶活性显着增加C大鼠（5%纤维素）由于IR治疗，而它显着下降或显示出下降的趋势，在5%Pec和20%HAS饮食喂养的大鼠。肝脏氧化型谷胱甘肽（GSSG）：总谷胱甘肽的比例显着增加IR大鼠维持在C饮食与假手术大鼠相比。另一方面，还原型谷胱甘肽（GSH）：总谷胱甘肽和GSH：GSSG比值显着下降。GSSG：由于IR处理而增加的总谷胱甘肽比率在HAS和Pec摄入时显著降低，而GSH：总谷胱甘肽和GSH：GSSG比率显著增加。IR大鼠喂食C饮食的肝窦被封闭，但IR大鼠喂食PEC饮食的肝窦与假手术大鼠相似。总之，我们发现，佩奇或HAS，这提高了H2的产生在大肠，减轻肝脏IR损伤。本研究还揭示了膳食纤维和RS的另一生理意义。
We investigated whether the feeding of high H-2-generating dietary fibre and resistant starch (RS) could suppress hepatic ischaemia-reperfusion (IR) injury, which results from oxidative stress, in rats fed a pectin (Pec) or high-amylose maize starch (HAS) diet. Male Sprague-Dawley rats were fed a control (C) diet, with or without Pec (0-5% Pec) or HAS (0-30% HAS) supplementation for 7 d. Portal H-2 concentration showed a significant dose-dependent increase with the amount of Pec or HAS supplementation. Plasma alanine and aspartate aminotransferase activities remarkably increased in the C rats (5% cellulose) due to IR treatment, while it decreased significantly or showed tendencies to decrease in 5% Pec and 20% HAS diet-fed rats. The hepatic oxidised glutathione (GSSG): total glutathione ratio increased significantly in IR rats maintained on the C diet compared with sham-operated rats. On the other hand, reduced glutathione (GSH):total glutathione and GSH: GSSG ratios decreased significantly. The GSSG: total glutathione ratio that increased due to IR treatment decreased significantly on HAS and Pec intake, while GSH: total glutathione and GSH: GSSG ratios increased significantly. Hepatic sinusoids of IR rats fed the C diet were occluded, but those of IR rats fed the Pec diet were similar to those in the sham-operated rats. In conclusion, we found that Pec or HAS, which enhance H2 generation in the large intestine, alleviated hepatic IR injury. The present study demonstrates another physiological significance of dietary fibre and RS.