Knockdown of a proliferation-inducing ligand (PRIL) suppresses the proliferation of gastric cancer cells.
Knockdown of a proliferation-inducing ligand (PRIL) suppresses the proliferation of gastric cancer cells.
复制标题
抑制增殖诱导配体（PRIL）可抑制胃癌细胞的增殖。
DOI：
10.7314/apjcp.2012.13.2.633
复制
发表时间：
2012
影响因子：
--
通讯作者：
Wei Li
中科院分区：
文献类型：
--
作者：
J. Cui;Yan Li;Chang Wang;C. Yao;Wei Li
目的
增殖诱导配体（proliferation-inducing ligand，PRIL）是新近发现的肿瘤坏死因子（tumor necrosis factor，TNF）家族成员，参与调节死亡配体诱导的细胞凋亡。在这里，我们研究了PRIL对人胃癌中与肿瘤进展相关的细胞特征的影响。
方法
构建含APRIL siRNA的重组慢病毒，感染胃癌细胞MGC803和SGC7901。MTT法[3-（4，5-dimethylthiazol-2-yl）-2，5-diphenyltetrazolium bromide]集落形成法和细胞周期分析法研究APRIL基因敲低对胃癌细胞增殖的影响。
结果
通过实时定量PCR证实，慢病毒感染细胞中的PRIL表达显著降低。在PRIL敲低的细胞中，MGC803和SGC7901细胞的细胞活力和集落形成显著受阻。此外，细胞周期被阻滞在G2/M期，阐明了siRNA抑制细胞增殖的机制。
结论
我们的研究表明PRIL具有促进细胞生长的功能，慢病毒介导的PRIL基因敲减可能成为胃癌治疗的一个有前景的策略。
PURPOSE
PRIL (proliferation-inducing ligand) is a newly identified member of the tumor necrosis factor (TNF) family and modulates death ligand-induced apoptosis. Here, we investigated the effect of PRIL on cellular characteristics relating to tumor progression in human gastric cancer.
METHOD
Recombinant lentivirus containing APRIL siRNA was constructed and then infected MGC803 and SGC7901 gastric cancer cells. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] colony formation and cell cycle analysis were used to study the effect of APRIL knockdown on gastric cancer cell proliferation.
RESULTS
PRIL expression in lentivirus infected cells was significantly reduced as evidenced by quantitative real-time PCR. Cell viability and colony formation of MGC803 and SGC7901 cells were significantly hampered in PRIL knock-down cells. Moreover, the cell cycle was arrested at G2/M phase, elucidating the mechanism underlying the inhibitory effect of siRNA on cell proliferation.
CONCLUSIONS
Our study indicated that PRIL functions in promoting cell growth, and lentivirus-mediated PRIL gene knockdown might be a promising strategy in the treatment of gastric cancer.
影响因子：
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作者：
Fish RJ;Kruithof EK
通讯作者：
Kruithof EK