Senseless and Daughterless confer neuronal identity to epithelial cells in the Drosophila wing margin
Senseless and Daughterless confer neuronal identity to epithelial cells in the Drosophila wing margin
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DOI：
10.1242/dev.02338
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发表时间：
2006-05-01
期刊：
影响因子：
4.6
通讯作者：
Bellen, HJ
中科院分区：
文献类型：
--
作者：
Jafar-Nejad, H;Tien, AC;Bellen, HJ
碱性螺旋-环-螺旋（bHLH）前神经蛋白Achaete和Scute与I类bHLH蛋白Daughterless合作，指定果蝇中大多数感觉刚毛的前体。然而，据报道，果蝇翅缘的机械感觉刚毛不受去除Achaete和Scute功能的突变的影响。事实上，这些器官的前神经基因是未知的。在这里，我们表明，锌指转录因子无意义的，与Daughterless一起，发挥proneural的作用，为翼缘mechanosensory前体，而Achaete和Scute所需的生存mechanosensory神经元和支持细胞在这些谱系。我们提供的证据表明，无意义和无女儿的物理相互作用和协同作用，在体内和转录测定。功能获得的研究表明，无感觉和无女儿是足够的，以产生胸部感觉器官（SO）的无毛鳞片基因复合体功能的情况下。然而，无意义的功能丧失克隆在胸部的分析牵连无意义的主要SO前体（pI）的选择，但在规范的pI后代。因此，尽管无意义蛋白和bHLH原神经蛋白在所有果蝇刚毛的发育过程中都被使用，但它们在这些器官的不同亚型中发挥着根本不同的作用。我们的数据表明，转录因子以外的bHLH蛋白也可以执行前神经功能的果蝇周围神经系统。
The basic helix-loop-helix (bHLH) proneural proteins Achaete and Scute cooperate with the class I bHLH protein Daughterless to specify the precursors of most sensory bristles in Drosophila. However, the mechanosensory bristles at the Drosophila wing margin have been reported to be unaffected by mutations that remove Achaete and Scute function. Indeed, the proneural gene(s) for these organs is not known. Here, we show that the zinc-finger transcription factor Senseless, together with Daughterless, plays the proneural role for the wing margin mechanosensory precursors, whereas Achaete and Scute are required for the survival of the mechanosensory neuron and support cells in these lineages. We provide evidence that Senseless and Daughterless physically interact and synergize in vivo and in transcription assays. Gain-of-function studies indicate that Senseless and Daughterless are sufficient to generate thoracic sensory organs (SOs) in the absence of achaete-scute gene complex function. However, analysis of senseless loss-of-function clones in the thorax implicates Senseless not in the primary SO precursor (pI) selection, but in the specification of pI progeny. Therefore, although Senseless and bHLH proneural proteins are employed during the development of all Drosophila bristles, they play fundamentally different roles in different subtypes of these organs. Our data indicate that transcription factors other than bHLH proteins can also perform the proneural function in the Drosophila peripheral nervous system.