Genome-wide analysis of expression modes and DNA methylation status at sense-antisense transcript loci in mouse.
Genome-wide analysis of expression modes and DNA methylation status at sense-antisense transcript loci in mouse.
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DOI：
10.1016/j.ygeno.2010.08.007
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发表时间：
2010-12
期刊：
影响因子：
4.4
通讯作者：
Yutaka Watanabe;K. Numata;S. Murata;Y. Osada;R. Saito;H. Nakaoka;Naoyuki Yamamoto;Kazufumi Watanabe;Hidemasa Kato;K. Abe;H. Kiyosawa
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文献类型：
--
作者：
Yutaka Watanabe;K. Numata;S. Murata;Y. Osada;R. Saito;H. Nakaoka;Naoyuki Yamamoto;Kazufumi Watanabe;Hidemasa Kato;K. Abe;H. Kiyosawa
正义反义转录本(SAT)的功能虽然广泛存在于哺乳动物基因组中，但在很大程度上是未知的。在这里，我们通过对12个小鼠组织的研究，分析了SATS的表达及其相关启动子DNA甲基化状态，以深入了解SATS的表达与DNA甲基化的关系。我们发现，在大多数组织中，正义和反义表达呈正相关。然而，在一些具有组织特异性表达的SAT中，当启动子DNA甲基化存在时，来自CpG岛启动子的转录本的表达水平很低。在这种情况下，其相反链转录物的表达水平恰好更高，特别是当它是Poly(A)阴性的时候。这些观察表明，尽管正义和反义基因同时表达的总趋势是这样的，但一些反义转录本与其对应的正义基因启动子甲基化具有协同表达。这种跨链关系并不是印迹基因的特权，但似乎在SAT中广泛存在。
The functionality of sense–antisense transcripts (SATs), although widespread throughout the mammalian genome, is largely unknown. Here, we analyzed the SATs expression and its associated promoter DNA methylation status by surveying 12 tissues of mice to gain insights into the relationship between expression and DNA methylation of SATs. We have found that sense and antisense expression positively correlate in most tissues. However, in some SATs with tissue-specific expression, the expression level of a transcript from a CpG island-bearing promoter is low when the promoter DNA methylation is present. In these circumstances, the expression level of its opposite-strand transcript, especially when it is poly(A)-negative was coincidentally higher. These observations suggest that, albeit the general tendency of sense–antisense simultaneous expression, some antisense transcripts have coordinated expression with its counterpart sense gene promoter methylation. This cross-strand relationship is not a privilege of imprinted genes but seems to occur widely in SATs.