First polar body morphology before ICSI is not related to embryo quality or pregnancy rate
First polar body morphology before ICSI is not related to embryo quality or pregnancy rate
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DOI：
10.1093/humrep/deh433
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发表时间：
2004-10-01
影响因子：
6.1
通讯作者：
Venturoli, S
中科院分区：
文献类型：
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作者：
Ciotti, PM;Notarangelo, L;Venturoli, S
背景：本研究旨在分析第一极体（1st PB）形态与受精率、卵裂率、胚胎质量、妊娠率和着床率的关系。方法：这是一项对 167 个连续周期进行 ICSI 辅助生殖的回顾性研究。在 ICSI 时对 596 个注射的卵母细胞进行第一次 PB 形态的评估，并将其编码为完整或片段。评估受精率、卵裂率、胚胎质量（三级）、妊娠率、着床率以及取卵与ICSI之间的时间间隔。为了验证体外培养的效果，随机抽取 180 个卵母细胞样本，对第 1 次 PB 形态进行两次检查（剥脱和 ICSI）。结果：第1次PB形态与受精率（P=0.703）、卵裂率（P=0.055）、胚胎质量（P=0.673）、妊娠率（P=0.201）和着床率（P=0.511）均无显着相关性。第一次 PB 碎片的频率与剥脱和 ICSI 之间的时间之间存在显着的正相关关系 (P=0.006)。在取出卵母细胞后5至7小时内注射而不是更早或更晚注射时，妊娠率显着更高（P=0.008）。结论：我们的数据表明，胚胎质量、妊娠率和着床率与第一次 PB 碎片无关。卵母细胞取出和 ICSI 之间的时间间隔应保持在 6 小时左右，以获得最佳结果。
BACKGROUND: The aim of this study was to analyse the relationship between the first polar body (1st PB) morphology and the fertilization rate, cleavage rate, embryo quality, pregnancy and implantation rate. METHODS: This was a retrospective study on 167 consecutive cycles undergoing assisted reproduction with ICSI. The 1st PB morphology was evaluated at the moment of ICSI in the 596 injected oocytes and it was coded as intact or fragmented. The fertilization rate, cleavage rate, embryo quality (three grades), pregnancy rate, implantation rate and the time elapsed between oocyte retrieval and ICSI were evaluated. The 1st PB morphology was checked twice (denudation and ICSI) in a random sample of 180 oocytes in order to verify the effect of the in vitro culture. RESULTS: No significant relationship was found between the 1st PB morphology and the fertilization rate (P=0.703), cleavage rate (P=0.055), embryo quality (P=0.673), pregnancy rate (P=0.201) and implantation rate (P=0.511). A significant positive relationship (P=0.006) was found between the frequency of the 1st PB fragmentation and the time elapsed between denudation and ICSI. The pregnancy rate was significantly higher (P=0.008) when oocytes were injected between 5 and 7 h after retrieval rather than earlier or later. CONCLUSIONS: Our data suggest that the embryo quality, pregnancy rate and implantation rate are not related to the 1st PB fragmentation. The time which elapses between the oocyte retrieval and ICSI should be maintained at similar to6 h in order to obtain optimal results.