Cross-linking and mass spectrometry methodologies to facilitate structural biology: finding a path through the maze.
Cross-linking and mass spectrometry methodologies to facilitate structural biology: finding a path through the maze.
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DOI：
10.1007/s10969-013-9160-z
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发表时间：
2013-09
期刊：
影响因子：
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通讯作者：
Adkins, Joshua N
中科院分区：
文献类型：
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作者：
Merkley, Eric D;Cort, John R;Adkins, Joshua N
多蛋白复合物，而不是单个蛋白质，构成了细胞生物大分子机制的很大一部分。了解这些复合物的结构和组织对于了解细胞功能至关重要。化学交联与质谱联用正在成为传统结构生物学方法的补充技术，并且可以为多种目的提供低分辨率结构信息，例如蛋白质复合物计算建模中的距离限制。在这篇综述中，我们讨论了化学交联质谱在生物学研究中成功应用的实验考虑因素，并重点介绍了最近文献中此类研究的三个例子。这些例子（以及许多其他例子）说明了化学交联质谱方法在促进大型且具有挑战性的复合物的结构分析方面的效用。
Multiprotein complexes, rather than individual proteins, make up a large part of the biological macromolecular machinery of a cell. Understanding the structure and organization of these complexes is critical to understanding cellular function. Chemical cross-linking coupled with mass spectrometry is emerging as a complementary technique to traditional structural biology methods and can provide low-resolution structural information for a multitude of purposes, such as distance constraints in computational modeling of protein complexes. In this review, we discuss the experimental considerations for successful application of chemical cross-linking-mass spectrometry in biological studies and highlight three examples of such studies from the recent literature. These examples (as well as many others) illustrate the utility of a chemical cross-linking-mass spectrometry approach in facilitating structural analysis of large and challenging complexes.