Functional Modulation of Receptor Proteins on Cellular Interface with Optogenetic System
Functional Modulation of Receptor Proteins on Cellular Interface with Optogenetic System
复制标题
光遗传学系统对细胞界面受体蛋白的功能调节
DOI：
10.1007/978-981-15-8763-4_15
复制
发表时间：
2021
影响因子：
--
通讯作者：
Ozawa Takeaki
中科院分区：
文献类型：
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作者：
Endo Mizuki;Ozawa Takeaki
在多细胞生物中，活细胞通过质膜上的蛋白质对细胞外的化学或物理刺激作出反应，从而相互协作，发挥协调的复杂功能。传统上，化学信号转导或机械转导是通过化学、遗传或物理扰动来研究的，然而，这些方法不能在高时空分辨率下操纵生物分子反应。相比之下，光遗传微扰方法的最新进展已经成功地控制了外部光的信号转导。这些方法实现了信号的时空扰动，提供了特定蛋白质的功能角色。在本章中，我们总结了调节受体蛋白功能的光遗传工具的最新进展。虽然大多数光遗传系统都是为了控制离子通道的电导而设计的，但本文重点介绍了参与化学转导或机械转导的其他膜蛋白。我们描述了用于光遗传系统的天然或人造光感受器蛋白的特性。然后，我们讨论了利用外界光控制受体蛋白功能的策略。展望了光遗传工具的发展前景。
In multicellular organisms, living cells cooperate with each other to exert coordinated complex functions by responding to extracellular chemical or physical stimuli via proteins on the plasma membrane. Conventionally, chemical signal transduction or mechano-transduction has been investigated by chemical, genetic, or physical perturbation; however, these methods cannot manipulate biomolecular reactions at high spatiotemporal resolution. In contrast, recent advances in optogenetic perturbation approaches have succeeded in controlling signal transduction with external light. The methods have enabled spatiotemporal perturbation of the signaling, providing functional roles of the specific proteins. In this chapter, we summarize recent advances in the optogenetic tools that modulate the function of a receptor protein. While most optogenetic systems have been devised for controlling ion channel conductivities, the present review focuses on the other membrane proteins involved in chemical transduction or mechano-transduction. We describe the properties of natural or artificial photoreceptor proteins used in optogenetic systems. Then, we discuss the strategies for controlling the receptor protein functions by external light. Future prospects of optogenetic tool development are discussed.