Analysis of microscopic bone properties in an osteoporotic sheep model: a combined biomechanics, FE and ToF-SIMS study
Analysis of microscopic bone properties in an osteoporotic sheep model: a combined biomechanics, FE and ToF-SIMS study
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DOI：
10.1098/rsif.2018.0793
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发表时间：
2019-02-01
影响因子：
3.9
通讯作者：
Deutsch, A.
中科院分区：
文献类型：
--
作者：
Mueller, R.;Henss, A.;Deutsch, A.
本研究探讨了绝经后骨质疏松症绵羊模型的骨质量特征。将绵羊假手术（n = 7）、卵巢切除（n = 6）、卵巢切除并用缺乏饮食处理（n = 8）或卵巢切除、用缺乏饮食和糖皮质激素注射处理（n = 7）。研究的重点是在组织水平上的微观特性。通过生物力学测试和数学建模相结合的方法，评价了骨质疏松骨的微观力学性能。通过压缩试验确定了样品的刚度和强度，并对应力状态进行了有限元分析。由此，确定组织水平的平均微观杨氏模量。应用显微计算机断层扫描和飞行时间二次离子质谱分析了羊椎骨骨小梁结构以及骨矿物质和胶原蛋白的分布。在骨质疏松绵羊模型中，在三联给药组中观察到纤维结构紊乱，但骨丢失仅以骨小梁数量和厚度减少以及皮质下降的形式发生，而残留骨的质量得到保留。保留的骨组织性质的绵羊模型允许骨强度的估计，其行为类似于人类的情况。
The present study deals with the characterization of bone quality in a sheep model of postmenopausal osteoporosis. Sheep were sham operated (n = 7), ovariectomized (n = 6), ovariectomized and treated with deficient diet (n = 8) or ovariectomized, treated with deficient diet and glucocorticoid injections (n = 7). The focus of the study is on the microscopic properties at tissue level. Microscopic mechanical properties of osteoporotic bone were evaluated by a combination of biomechanical testing and mathematical modelling. Sample stiffness and strength were determined by compression tests and finite-element analysis of stress states was conducted. From this, an averaged microscopic Young's modulus at tissue level was determined. Trabecular structure as well as mineral and collagen distribution in samples of sheep vertebrae were analysed by micro-computed tomography and time-of-flight secondary ion mass spectrometry. In the osteoporotic sheep model, a disturbed fibril structure in the triple treated group was observed, but bone loss only occurred in form of reduced trabecular number and thickness and cortical decline, while quality of the residual bone was preserved. The preserved bone tissue properties in the osteoporotic sheep model allowed for an estimation of bone strength which behaves similar to the human case.