A HUMAN LYMPHOCYTE-ASSOCIATED ANTIGEN INVOLVED IN CELL-MEDIATED LYMPHOLYSIS
A HUMAN LYMPHOCYTE-ASSOCIATED ANTIGEN INVOLVED IN CELL-MEDIATED LYMPHOLYSIS
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DOI：
10.1002/eji.1830130305
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发表时间：
1983-01-01
影响因子：
5.4
通讯作者：
MCMICHAEL, AJ
中科院分区：
文献类型：
--
作者：
HILDRETH, JEK;GOTCH, FM;MCMICHAEL, AJ
人淋巴细胞功能抗原 (HLFA) 是由 2 种单克隆抗体 MHM23 和 MHM24 定义的细胞表面蛋白。它存在于 B 淋巴细胞和 T 淋巴细胞上，但后者的含量更高。两种抗体均从放射性标记的 HSB-2（人淋巴母细胞）细胞中沉淀抗原，该细胞在十二烷基硫酸钠聚丙烯酰胺凝胶电泳上以 180 和 94 kDa [千道尔顿] 的速度呈两条链运行。两种抗体均不抑制另一种抗体的结合，表明不同的表位被识别。两种抗体均能抑制细胞毒性 T 淋巴细胞对流感病毒感染和 Epstein-Barr 病毒转化的靶细胞的 HLA 限制性裂解。阻断发生在效应细胞水平且存在亚饱和浓度的抗体的情况下。两种试剂还抑制自然杀伤细胞介导的 K562 [人类白血病]细胞的裂解。这些阻断作用不同于单克隆抗 HLA ABC 和抗抑制性细胞毒性 T 细胞抗体的抑制作用，后者在以饱和量存在时仅抑制 HLA 限制性裂解。 HLFA 可能参与杀伤细胞的非特异性粘附或裂解功能，而不是特异性抗原识别。
Human lymphocyte function antigen (HLFA) is a cell surface protein defined by 2 monoclonal antibodies MHM23 and MHM24. It is present on both B and T lymphocytes but in greater amounts on the latter. Both antibodies precipitated antigen, from radiolabeled HSB-2 [human lymphoblastoid] cells, which ran as 2 chains on sodium dodecyl sulfate polyacrylamide gel electrophoresis at 180 and 94 kDa [kilodalton]. Neither antibody inhibited binding of the other, indicating that distinct epitopes were recognized. Both antibodies were shown to inhibit HLA-restricted lysis of influenza virus-infected and Epstein-Barr virus-transformed target cells by cytotoxic T lymphocytes. Blocking occurred at the level of the effector cells and in the presence of subsaturating concentrations of antibody. Both reagents also inhibited lysis of K562 [human leukemia] cells, mediated by natural killer cells. These blocking effects differ from the inhibitory effects of monoclonal anti-HLA ABC and anti-suppressor cytotoxic T cell antibodies which inhibit only HLA-restricted lysis when present in saturating amounts. HLFA may be involved in the nonspecific adherence or lytic functions of killer cells rather than specific antigen recognition.