A modified colorimetric method for phytic acid analysis in soybean
A modified colorimetric method for phytic acid analysis in soybean
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DOI：
10.2135/cropsci2007.03.0122
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发表时间：
2007-09-01
期刊：
影响因子：
2.3
通讯作者：
Buss, G. R.
中科院分区：
文献类型：
--
作者：
Gao, Y.;Shang, C.;Buss, G. R.
需要一种定量、可重复且高效的植酸测定程序来筛选育种群体并支持大豆的遗传研究 [Glycine max (L.) Merr.]。本研究的目的是修改比色韦德试剂方法，并比较这种新方法测定大豆种子植酸含量的准确性和适用性与三种成熟的植酸测定方法：阴离子交换柱（AEC）、高效液相色谱（HPLC）和P-31核磁共振（NMR）。重复测量低植酸大豆突变体 CX1834-1-6 的 CV 范围为 1.8% 至 4.2% (n = 5)，表明结果精确且可重复。通过该方法测定的 42 个大豆基因型的植酸含量与 AEC、HPLC 和 NMR 的测量结果显示出 93.7% 至 96.6% 的相关性。根据协方差分析，以无机磷含量为预测因子，用四种测定方法分析的给定样品中植酸磷含量可以在(x = 0.01水平上用四种线性回归模型进行估计。与HPLC、AEC和P-11 NMR相比，这种改进的比色方法更简单、成本更低，适合测定大量样品，使其能够有效应用于低植酸大豆的育种和遗传研究。
A quantitative, reproducible, and efficient phytic acid assay procedure is needed to screen breeding populations and support genetic studies in soybeans [Glycine max (L.) Merr.]. The objective of this study was to modify the colorimetric Wade reagent method and compare the accuracy and applicability of this new method in determining seed phytic acid content in soybean with three well-established phytic acid assay methods: anion exchange column (AEC), high-performance liquid chromatography (HPLC), and P-31 nuclear magnetic resonance (NMR). The CV for repeated measurements of a low phytic acid soybean mutant, CX1834-1-6, ranged from 1.8 to 4.2% (n = 5), indicating the results were precise and reproducible. Phytic acid content of 42 soybean genotypes as determined by this method showed a correlation of 93.7 to 96.6% with the measurements by AEC, HPLC, and NMR. According to analysis of covariance, using inorganic P content as a predictor, phytic acid P content in a given sample analyzed by the four assay methods can be estimated with four linear regression models at the (x = 0.01 level. Compared with HPLC, AEC, and P-11 NMR, this modified colorimetric method is simpler and less expensive for assaying a large number of samples, allowing its effective application in breeding and genetic studies of low phytic acid soybean.