Intravenous administration of mannosylated cationic liposome/NFκB decoy complexes effectively prevent LPS‐induced cytokine production in a murine liver failure model
Intravenous administration of mannosylated cationic liposome/NFκB decoy complexes effectively prevent LPS‐induced cytokine production in a murine liver failure model
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DOI：
10.1016/j.febslet.2006.05.059
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发表时间：
2006-06
期刊：
影响因子：
3.5
通讯作者：
Y. Higuchi;S. Kawakami;M. Oka;Y. Yabe;F. Yamashita;M. Hashida
中科院分区：
文献类型：
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作者：
Y. Higuchi;S. Kawakami;M. Oka;Y. Yabe;F. Yamashita;M. Hashida
本研究旨在通过甘露糖基化阳离子脂质体（Man-liposomes）选择性地将核因子κB（NFκB）诱骗物（decoy）递送至肝非实质细胞（NPC），以抑制内毒素诱导的细胞因子产生和肝损伤。本研究观察了静脉注射Man-liposomes/NFκB decoy复合物后的分布、对细胞因子产生的抑制作用以及对ALT/AST的影响。人脂质体/[32 P] NFκB诱饵复合物主要聚集在肝脏，优先聚集在NPC中。在脂多糖诱导的小鼠肝衰竭模型中，Man-脂质体复合物可有效地降低肿瘤坏死因子-α（TNFα）、IFNγ、IL 1-β、ALT和AST的产生。而阳离子脂质体复合物或半乳糖基化的阳离子脂质体复合物不能抑制TNFα的产生。
The purpose of this study was to inhibit endotoxin induced cytokines production and liver injury by liver non-parenchymal cell (NPC) selective delivery of nuclear factor κB (NFκB) decoy using mannosylated cationic liposomes (Man-liposomes). In this study, we examined the distribution, inhibitory effect on cytokines production and ALT/AST of intravenously injected Man-liposome/NFκB decoy complex. Man-liposome/[32P] NFκB decoy complexes mostly accumulated in the liver, preferentially in NPC. In a murine lipopolysaccharide-induced liver failure model, the production of tumor necrosis factor-α (TNFα), IFNγ, IL1-β, ALT and AST were effectively reduced by Man-liposome complexes. However, cationic or galactosylated cationic liposome complexes could not inhibit TNFα production.