DENDRITIC SPINES AS INDIVIDUAL NEURONAL COMPARTMENTS FOR SYNAPTIC CA2+ RESPONSES
DENDRITIC SPINES AS INDIVIDUAL NEURONAL COMPARTMENTS FOR SYNAPTIC CA2+ RESPONSES
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DOI：
10.1038/354073a0
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发表时间：
1991-11-07
期刊：
影响因子：
64.8
通讯作者：
CONNOR, JA
中科院分区：
文献类型：
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作者：
MULLER, W;CONNOR, JA
神经元树突上的突触后棘可能是参与突触可塑性1-6的Ca2+激活过程的离散生化室，这是一个被广泛提出的概念，但尚未得到实验证明。通过对海马CA3神经元切片的显微荧光测定，我们在这里显示，在突触前对联合/联合纤维的弱刺激下，Ca2+在单个突触后棘中积累，而不是在亲本树突中积累。更强的刺激也会促进树突的变化。nmda受体拮抗剂AP-5阻断了脊柱中Ca2+的变化。持续陡峭的Ca2+梯度之间的单棘和亲本树突，往往持续几分钟，发展与反复刺激。观察到的区隔化允许特异性7,8，合作性9和联想性10-14显示的记忆模型，如长期增强。
THE possibility that postsynaptic spines on neuronal dendrites are discrete biochemical compartments for Ca2+-activated processes involved in synaptic plasticity 1-6 is a widely proposed concept that has eluded experimental demonstration. Using microfluorometry on CA3 neurons in hippocampal slices, we show here that with weak presynaptic stimulation of associative/commissural fibres, Ca2+ accumulates in single postsynaptic spines but not in the parent dendrite. Stronger stimulation also promotes changes in dendrites. The NMDA-receptor antagonist AP-5 blocks changes in Ca2+ in spines. Sustained steep Ca2+ gradients between single spines and the parent dendrite, often lasting several minutes, develop with repeated stimulation. The observed compartmentalization allows for the specificity 7,8, cooperativity 9 and associativity 10-14 displayed by memory models such as long-term potentiation.