Mixed culture of oleaginous yeast Rhodotorula glutinis and microalga Chlorella vulgaris for lipid production from industrial wastes and its use as biodiesel feedstock
Mixed culture of oleaginous yeast Rhodotorula glutinis and microalga Chlorella vulgaris for lipid production from industrial wastes and its use as biodiesel feedstock
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DOI：
10.1016/j.nbt.2011.01.004
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发表时间：
2011-07-01
影响因子：
5.4
通讯作者：
Niyomdecha, Rujira
中科院分区：
文献类型：
--
作者：
Cheirsilp, Benjamas;Suwannarat, Warangkana;Niyomdecha, Rujira
利用产油酵母Rhodotorula seduis和小球藻Chlorella vulgaris混合培养提高工业废弃物中油脂的产量。这包括海产加工厂的污水和甘蔗厂的糖蜜。在混合培养中，酵母生长速度快，油脂产量高于纯培养。这可能是因为微生物作为酵母的氧气发生器，而酵母为微生物提供二氧化碳，两者都进行脂质的生产。结果表明，混合培养的最佳产脂条件为：酵母菌与酵母菌的比例为1：1，初始pH值为5.0，糖蜜浓度为1%，摇床转速为200 rpm，光照强度为5.0 klux，光照时间为16：8 h。在这些条件下，培养5天后获得最高生物量4.63 +/- 0.15 g/L和脂质产量2.88 +/- 0.16 g/L。此外，酵母和微藻脂质的植物油样脂肪酸组成表明它们作为生物柴油原料的高潜力。
A mixed culture of oleaginous yeast Rhodotorula glutinis and microalga Chlorella vulgaris was performed to enhance lipid production from industrial wastes. These included effluent from seafood processing plant and molasses from sugar cane plant. In the mixed culture, the yeast grew faster and the lipid production was higher than that in the pure cultures. This could be because microalga acted as an oxygen generator for yeast, while yeast provided CO2 to microalga and both carried out the production of lipids. The optimal conditions for lipid production by the mixed culture were as follows: ratio of yeast to microalga at 1:1; initial pH at 5.0; molasses concentration at 1%; shaking speed at 200 rpm; and light intensity at 5.0 klux under 16:8 hours light and dark cycles. Under these conditions, the highest biomass of 4.63 +/- 0.15 g/L and lipid production of 2.88 +/- 0.16 g/L were obtained after five days of cultivation. In addition, the plant oil-like fatty acid composition of yeast and microalgal lipids suggested their high potential for use as biodiesel feedstock.