Binding of DNA with Rhodamine B: Spectroscopic and molecular modeling studies
Binding of DNA with Rhodamine B: Spectroscopic and molecular modeling studies
复制标题
DOI：
10.1016/j.dyepig.2013.05.028
复制
发表时间：
2013-11-01
影响因子：
4.5
通讯作者：
Mukhopadhyay, Subrata
中科院分区：
文献类型：
--
作者：
Islam, Md. Maidul;Chakraborty, Maharudra;Mukhopadhyay, Subrata
用生物物理技术和分子对接方法研究了罗丹明B与小牛胸腺DNA的结合。圆二色性研究表明，CT DNA的构象发生了适度的变化，并与罗丹明B结合后发生了解旋。这种约束是合作性质的。根据Benesi Hidebrand图评价的总体结合常数在10（3）M-1范围内。盐依赖性结合数据表明，结合自由能依赖于盐浓度。热力学研究表明，负焓变和正熵变都有利于键合过程。分子对接计算和猝灭实验证实了染料结合在CT DNA的小沟中。这些结果进一步推进了我们对这些类型的染料与核酸相互作用的分子方面的知识。(C)2013爱思唯尔有限公司保留所有权利。
Binding of Rhodamine B to Calf thymus DNA (CT DNA) was studied using various biophysical techniques and molecular docking method. Circular dichroic studies revealed that conformation of CT DNA changed moderately and unwind on binding with Rhodamine B. The binding was cooperative in nature. The overall binding constant, evaluated from Benesi Hidebrand plot was seen to be in the range of 10(3) M-1. Salt dependence binding data showed that the binding free energy depends on salt concentration. From the thermodynamics studies, it was concluded that binding process is favored by both negative enthalpy change and positive entropy change. Molecular docking calculations and Quenching experiment confirmed that the dye binds in the minor groove of CT DNA. These results further advance our knowledge on the molecular aspects on the interaction of these types of dyes to nucleic acids. (C) 2013 Elsevier Ltd. All rights reserved.