Antibacterial activity of plants used in traditional medicines of Ghana with particular reference to MRSA
Antibacterial activity of plants used in traditional medicines of Ghana with particular reference to MRSA
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DOI：
10.1016/j.jep.2007.11.005
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发表时间：
2008-02-28
影响因子：
5.4
通讯作者：
Humber, David P.
中科院分区：
文献类型：
--
作者：
Pesewu, George A.;Cutler, Ronald R.;Humber, David P.
民族药物学相关性：在加纳共和国阿克瓦皮姆北区进行的民族植物学调查中，发现了25种植物。在传统医学中用于治疗皮肤病和/或治疗抗微生物（病毒、细菌或原生动物）感染。研究目的：研究加纳传统药物的抗菌活性，特别是抗耐甲氧西林金黄色葡萄球菌（MRSA）的活性。材料和方法：制备了这些植物的氯仿、乙醇和水提取物（包括使用胃囊），并采用琼脂孔扩散试验、MIC试验和MBC试验考察了它们的抗菌活性。结果：13种植物提取物对MRSA、甲氧西林敏感金黄色葡萄球菌（MSSA）、化脓性链球菌、大肠杆菌、铜绿假单胞菌和寻常变形杆菌的生长均有抑制作用。这13种植物中11种的提取物也抑制了另外14种MRSA临床分离株中的3种或更多的生长。苜蓿水提物对21株MRSA均有抑菌活性，MIC值为1.6 ~ 3.1 mg ml(-1)， MBC值为6.3 ~ 12.5 mg ml（-1）。结论：首次报道了治疗皮肤感染的传统植物——松果、吐虫和龙葵叶提取物的抑菌活性。也用于治疗伤口的堇青花提取物具有最广泛的抗菌活性。2007爱思唯尔爱尔兰有限公司版权所有。
Ethnopharmacological relevance: : In an ethno botanical survey carried out in the Akwapim-North district of the Republic of Ghana, 25 plant species. used in traditional medicine to treat skin disease and/or to treat antimicrobial (viral, bacterial or protozoan) infections were identified.Aim of Study : To investigate the antimicrobial activity of traditional Ghanaian medicines with special interest in anti-methicillin-resistant Staphylococcus aureus (MRSA) activity.Materials and methods: : Chloroform, ethanol and aqueous extracts (including use of a Stomacher) of these plants were prepared and agar-well diffusion tests, MIC's and MBC's were used to investigate antimicrobial activity.Results: Extracts of 13 plant species inhibited the growth of one or more of the following bacteria: MRSA, methicillin-sensitive Staphylococcus aureus (MSSA), Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris. Extracts from 11 of these 13 plant species also inhibited the growth of three or more of 14 additional clinical isolates of MRSA. Aqueous extracts of Alchornea cordifolia were active against all 21 bacterial strains tested and showed the highest levels of antibacterial activity overall with MIC's against MRSA in the range of 1.6-3.1 mg ml(-1) and MBC's in the range of 6.3-12.5 mg ml(-1).Conclusions: : The presence of antibacterial activity in extracts of Elaeophorbia drupifera, Rauwolfia vomitoria and the leaves of Solanum verbascifolium, plants traditionally used to treat skin infections, are reported for the first time. Extracts from Alchornea cordifolia, also used to treat wounds, had the widest spectrum of antibacterial activity. (C) 2007 Elsevier Ireland Ltd. All rights reserved.