Epidemiology of Staphylococcus aureus colonization in nursing home residents
Epidemiology of Staphylococcus aureus colonization in nursing home residents
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DOI：
10.1086/586751
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发表时间：
2008-05-01
影响因子：
11.8
通讯作者：
Bradley, Suzanne F.
中科院分区：
文献类型：
--
作者：
Mody, Lona;Kauffman, Carol A.;Bradley, Suzanne F.
背景资料。我们试图描述金黄色葡萄球菌定植(特别是鼻外定植)的临床和分子流行病学特征，并确定社区疗养院出现社区相关性耐甲氧西林金黄色葡萄球菌(MRSA)的程度。这项研究共招募了来自密歇根州东南部14家养老院的213名居民，无论是否有留置装置。样本取自鼻孔、口咽、腹股沟、肛周、伤口和肠道喂养管部位。用标准微生物学方法鉴定甲氧西林敏感的金黄色葡萄球菌和MRSA。分子流行病学方法包括脉冲场凝胶电泳法、聚合酶链式反应检测潘通-瓦伦丁杀白素、SCCmec和agr分型。131名居民(62%)感染金黄色葡萄球菌(86人感染耐甲氧西林金黄色葡萄球菌)。有留置装置的105名居民中有80人(76%)发生了金黄色葡萄球菌定植，108名无留置装置的居民中有51人(47%)发生了金黄色葡萄球菌定植。在86名感染MRSA的居民中，NARES培养P
Background. We sought to characterize the clinical and molecular epidemiologic characteristics of Staphylococcus aureus colonization ( especially extranasal colonization) and to determine the extent to which community-associated methicillin- resistant S. aureus ( MRSA) has emerged in community nursing homes.Methods. The study enrolled a total of 213 residents, with or without an indwelling device, from 14 nursing homes in southeastern Michigan. Samples were obtained from the nares, oropharynx, groin, perianal area, wounds, and enteral feeding tube site. Standard microbiologic methods were used to identify methicillin- susceptible S. aureus and MRSA. Molecular epidemiologic methods included pulsed- field gel electrophoresis, PCR detection of Panton- Valentine leukocidin, and SCCmec and agr typing.Results. One hundred thirty- one residents ( 62%) were colonized with S. aureus ( MRSA colonization in 86). S. aureus colonization occurred in 80 ( 76%) of 105 residents with indwelling devices and in 51 ( 47%) of 108 residents without indwelling devices (). Of the 86 residents who were colonized with MRSA, nares culture P