The differential proteome profile of stomach cancer: Identification of the biomarker candidates
The differential proteome profile of stomach cancer: Identification of the biomarker candidates
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DOI：
10.3727/0965040042380441
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发表时间：
2004-01-01
影响因子：
3.1
通讯作者：
Kim, HW
中科院分区：
文献类型：
--
作者：
Jang, JS;Cho, HY;Kim, HW
通过比较蛋白质组学分析，我们寻找人胃腺癌组织和配对的周围正常组织的特征性改变。采用基于基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF）的肽质量指纹图谱和数据库检索对所选差异蛋白点进行鉴定。我们确定了18例胃癌组织与正常对照组相比的蛋白质改变，包括8种蛋白质水平升高，包括14-3-3 zeta、钙周期蛋白、角蛋白、载脂蛋白A-1前体、蛋白酶体激活物复合物亚基、核苷二磷酸激酶、烟酰N-甲基转移酶和吡哆醛激酶。5种蛋白质（CA 11、抑制素、过氧化物酶氧还蛋白4、血清淀粉样蛋白P组分和NADH-泛醌氧化还原酶23 kDa亚基）降低。这些数据对于鉴定参与胃癌癌变过程的差异表达蛋白，为开发诊断和治疗工具提供生物标志物候选者是有价值的。
By comparative proteome analysis we searched for characteristic alterations of human stomach adenocarcinoma tissue and paired surrounding normal tissue. Selected differential protein spots were identified with peptide mass fingerprinting based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) and database searching. We identified protein alterations in 18 stomach cancer tissues compared with normal controls, comprising elevated levels of eight proteins, including 14-3-3 zeta, calcyclin, keratin, apolipoprotein A-1 precursor, proteasome activator complex subunit, nucleoside diphosphate kinase, nicotinanride N-methyltransferase, and pyridoxal kinase. Five proteins (CA11, prohibitin, peroxiredoxin 4, serum amyloid P component, and NADH-ubiquinone oxidoreductase 23 kDa subunit) were decreased. These data are valuable for identification of differentially expressed proteins involved in stomach cancer carcinogenesis, providing biomarker candidates to develop diagnostic and therapeutic tools.