Evaluation of a Norwegian-developed ELISA to determine microcystin concentrations in fresh water
Evaluation of a Norwegian-developed ELISA to determine microcystin concentrations in fresh water
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DOI：
10.2166/ws.2018.118
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发表时间：
2019-05-01
期刊：
影响因子：
1.7
通讯作者：
Myburgh, J. G.
中科院分区：
文献类型：
--
作者：
Botha, C. J.;Laver, P. N.;Myburgh, J. G.
蓝藻以其在非洲河流或水坝中广泛和高度可见的开花而闻名。最重要的蓝藻之一是铜绿微囊藻，它可以合成各种可能影响人类和动物健康的微囊藻毒素。因此，准确和有效地检测水中微囊藻毒素对公众和兽医卫生至关重要。两种酶联免疫吸附测定法（ELISA），一种市售ELISA试剂盒（Abraxis）和一种新开发的挪威ELISA（假定更便宜和更可靠）用于检测南非淡水中的微囊藻毒素。每月在哈特比斯波特大坝和鳄鱼繁殖大坝两个地点采集水样。2015年1月，哈特比斯波特大坝微囊藻毒素浓度极高（超过360 μ g L-1），而鳄鱼繁殖坝微囊藻毒素浓度在2015年3 - 4月达到峰值。两种elisa在分析水样“原样”和树脂吸附和甲醇提取时呈正相关。然而，在对水样进行树脂吸附和甲醇萃取后，两者之间的相关性要强得多。这些结果表明，这两种elisa提供了可比较的结果。如果挪威开发的酶联免疫吸附试验能够包装并作为用户友好的试剂盒提供，它可以成功地用于监测非洲淡水水体中微囊藻毒素浓度的监测规划。
Cyanobacteria are known for their extensive and highly visible blooms in rivers or dams in Africa. One of the most important cyanobacteria is Microcystis aeruginosa which can synthesise various microcystins that may affect the health of humans and animals. Accurate and efficient detection of microcystins in water is thus important for public and veterinary health. Two enzyme-linked immunosorbent assays (ELISA), a commercially-available ELISA kit (Abraxis) and a newly-developed Norwegian ELISA (putatively cheaper and more robust) were used to detect microcystins in fresh water in South Africa. Water samples were collected monthly at two sites, the Hartbeespoort Dam and a crocodile breeding dam. Extremely high microcystin concentrations (exceeding 360 mu g L-1) were detected in the Hartbeespoort Dam during January 2015, whereas the microcystin concentrations in the crocodile breeding dam peaked during March-April 2015. Both ELISAs were positively correlated when analysing water samples 'as is' and following resin adsorption and methanol extraction. However, following resin adsorption and methanol extraction of the water samples, the correlation between the two assays was much stronger. These results suggests that the two ELISAs provide comparable results. If the Norwegian-developed ELISA can be packaged and made available as a user-friendly kit, it could be used successfully in surveillance programmes to monitor microcystin concentrations in fresh water bodies in Africa.