Myocardial antioxidant and oxidative stress changes due to sex hormones
Myocardial antioxidant and oxidative stress changes due to sex hormones
复制标题
DOI：
10.1590/s0100-879x2002000900008
复制
发表时间：
2002-09-01
影响因子：
2.3
通讯作者：
Singal, P.
中科院分区：
文献类型：
--
作者：
Barp, J.;Araújo, A.S.R.;Singal, P.
本研究的目的是研究在生理性激素浓度的存在下和去势后，雄性和雌性大鼠心肌抗氧化和氧化应激的变化。将24只9周龄Wistar大鼠分为4组，每组6只动物：1）假手术雌性，2）去势雌性，3）假手术雄性和4）去势雄性。当通过放射免疫测定法测量睾酮和雌激素水平时，在去势组和对照组（雄性和雌性）之间观察到显著差异，证明去势成功。任何组的预后和过氧化氢酶水平均无变化。对照组雄性大鼠谷胱甘肽过氧化物酶水平高于雌性大鼠（50%），超氧化物歧化酶（SOD，14%）水平低于雌性大鼠。与其他组相比，对照组雌性动物的SOD水平升高。去势后，SOD活性下降了29%，在女性组和14%，在男性组相比，各自的控制。通过两种不同的方法评估脂质过氧化（LPO）以评价心肌膜的氧化损伤，即，TBARS和化学发光。通过TBARS（360%）和化学发光（46%）两种方法评价时，男性对照组的LPO高于女性对照组。通过TBARS测定，去势诱导雌性动物心肌损伤增加200%，通过化学发光测定，增加20%。在雄性中，去势没有改变LPO水平。这些数据表明，雌激素可能对心肌具有抗氧化作用，而睾酮则没有。
The purpose of the present study was to examine myocardial antioxidant and oxidative stress changes in male and female rats in the presence of physiological sex hormone concentrations and after castration. Twenty-four 9-week-old Wistar rats were divided into four groups of 6 animals each: 1) sham-operated females, 2) castrated females, 3) sham-operated males, and 4) castrated males. When testosterone and estrogen levels were measured by radioimmunoassay, significant differences were observed between the castrated and control groups (both males and females), demonstrating the success of castration. Progesterone and catalase levels did not change in any group. Control male rats had higher levels of glutathione peroxidase (50%) and lower levels of superoxide dismutase (SOD, 14%) than females. Control females presented increased levels of SOD as compared to the other groups. After castration, SOD activity decreased by 29% in the female group and by 14% in the male group as compared to their respective controls. Lipid peroxidation (LPO) was assessed to evaluate oxidative damage to cardiac membranes by two different methods, i.e., TBARS and chemiluminescence. LPO was higher in male controls compared to female controls when evaluated by both methods, TBARS (360%) and chemiluminescence (46%). Castration induced a 200% increase in myocardial damage in females as determined by TBARS and a 20% increase as determined by chemiluminescence. In males, castration did not change LPO levels. These data suggest that estrogen may have an antioxidant role in heart muscle, while testosterone does not.