Nuclear Magnetic Resonance Measurement of Metabolic Flux Using 13C and 1H Signals.
Nuclear Magnetic Resonance Measurement of Metabolic Flux Using 13C and 1H Signals.
复制标题
使用 13C 和 1H 信号进行代谢通量的核磁共振测量。
DOI：
10.1007/978-1-4939-9488-5_3
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Merritt,MatthewE
中科院分区：
文献类型：
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作者：
Ragavan,Mukundan;Merritt,MatthewE
代谢通量建模是标准代谢组学/代谢组学在生物系统中常规应用的补充技术。在这里，我们概述了以核磁共振为主要分析技术的碳-13基同位素分析。同位素分析得出三羧酸循环周转的相对速率。如果将这些指标与二氧化碳消耗量归一化，就可以推断出绝对比率。本综述主要针对的生物系统是细胞培养。
Metabolic flux modeling is a complementary technique to standard metabolomics/metabonomics as routinely practiced in biological systems. Here we outline carbon-13-based isotopomer analysis using nuclear magnetic resonance as the primary analytical technique. Isotopomer analysis produces relative rates of tricarboxylic acid cycle turnover. If these measures are normalized to O2consumption, absolute rates can be inferred. The primary biological system targeted in this review is cell culture.