Morphological description of limbal epithelium: searching for stem cells crypts in the dog, cat, pig, cow, sheep and horse
Morphological description of limbal epithelium: searching for stem cells crypts in the dog, cat, pig, cow, sheep and horse
复制标题
DOI：
10.1007/s11259-017-9676-y
复制
发表时间：
2017-06-01
影响因子：
2.2
通讯作者：
Iacopetti, I.
中科院分区：
文献类型：
--
作者：
Patruno, M.;Perazzi, A.;Iacopetti, I.
角膜为眼睛提供保护和透明度，从而获得最佳清晰度。在某些病理条件下，由于存在于角膜和巩膜之间的过渡区域（利姆布斯）水平处的干细胞储库，角膜能够再生。兽医学中的角膜细胞疗法由于缺乏关于角膜缘区域的解剖学、干细胞的假定存在及其在家养物种中的鉴定的知识而受到限制。本研究的目的是使用组织学切片的光学显微镜观察，概述某些兽医重要种属中巩膜-角膜交界区和结膜-角膜交界区的主要独特结构特征。将所得数据与人类角膜进行比较，这些角膜适应了已经用于通过特定细胞标志物识别干细胞的方案。我们测试了角膜基底细胞中Delta Np 63 α亚型的表达，试图将分布特征与高度增殖性转换区域相关联。从这项研究中获得的结果代表了在不同动物中鉴定角膜干细胞储库的第一步。
The cornea provides protection and transparency to the eye, allowing an optimal sharpness view. In some pathological conditions the cornea is able to regenerate thanks to the presence of a stem cells reservoir present at the level of the transition area between cornea and sclera (limbus). Corneal cell therapies in Veterinary Medicine are really limited due to the lacking of knowledge about the anatomy of the limbal area, the putative presence of stem cells and their identification in domestic species. The aim of this study was to provide an overview of the main distinctive structural features of the sclero-corneal junction and conjunctival-corneal junction areas in some species of veterinary importance, using optic microscope observations of histological sections. The resulting data were compared with cornea from humans adapting protocols already used to identify stem cells by means of a specific cellular marker. We tested the expression of Delta Np63 alpha isoform in the cornea basal cells, trying to correlate the distribution profile with areas of highly proliferative turnover. The results obtained from this study represent a first step towards the identification of a corneal stem cells reservoir in different animals.